Evaluation of Biocompatibility of a Standardized Extract of Agave angustifolia Haw in Human Dermal Fibroblasts

Agave angustifolia Haw has been used as an ethnobotanical plant, for the treatment of various diseases. In addition, different biological activities attributed to its active compounds have been reported, but their biocompatibility has been little explored. The objective of this research work was to determine the in vitro cytotoxicity of the ethanolic extract of A. angustifolia. Methodology: Six different concentrations (0.01, 0.1, 1, 10, 100, 500 μg/ml) of the ethanolic extract of A. angustifolia were tested for 48 h of contact with the human dermal fibroblasts (HDF) to evaluate its cytotoxicity using the sulforhodamine (SRB) colorimetric test. On the contrary, HDF with concentrations of 100 and 500 μg/ml showed a high percentage of viability over the control group, more evident in the concentration of 500 μg/ml, which had a 24% increase in the fibroblast population. Conclusions: The ethanolic extract from A. angustifolia obtained by MAE, there was no evidence of cytotoxicity. It was able to be considered as a biocompatible material. Rather, it was shown to promote fibroblast proliferation.