TY - GEN
T1 - Evaluation of Biocompatibility of a Standardized Extract of Agave angustifolia Haw in Human Dermal Fibroblasts
AU - López-Salazar, Herminia
AU - Tapia, Jesús Santa Olalla
AU - Camacho-Díaz, Brenda Hildeliza
AU - Ocampo, Martha L.Arenas
AU - Jiménez-Aparicio, Antonio R.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2022
Y1 - 2022
N2 - Agave angustifolia Haw has been used as an ethnobotanical plant, for the treatment of various diseases. In addition, different biological activities attributed to its active compounds have been reported, but their biocompatibility has been little explored. The objective of this research work was to determine the in vitro cytotoxicity of the ethanolic extract of A. angustifolia. Methodology: Six different concentrations (0.01, 0.1, 1, 10, 100, 500 μg/ml) of the ethanolic extract of A. angustifolia were tested for 48 h of contact with the human dermal fibroblasts (HDF) to evaluate its cytotoxicity using the sulforhodamine (SRB) colorimetric test. On the contrary, HDF with concentrations of 100 and 500 μg/ml showed a high percentage of viability over the control group, more evident in the concentration of 500 μg/ml, which had a 24% increase in the fibroblast population. Conclusions: The ethanolic extract from A. angustifolia obtained by MAE, there was no evidence of cytotoxicity. It was able to be considered as a biocompatible material. Rather, it was shown to promote fibroblast proliferation.
AB - Agave angustifolia Haw has been used as an ethnobotanical plant, for the treatment of various diseases. In addition, different biological activities attributed to its active compounds have been reported, but their biocompatibility has been little explored. The objective of this research work was to determine the in vitro cytotoxicity of the ethanolic extract of A. angustifolia. Methodology: Six different concentrations (0.01, 0.1, 1, 10, 100, 500 μg/ml) of the ethanolic extract of A. angustifolia were tested for 48 h of contact with the human dermal fibroblasts (HDF) to evaluate its cytotoxicity using the sulforhodamine (SRB) colorimetric test. On the contrary, HDF with concentrations of 100 and 500 μg/ml showed a high percentage of viability over the control group, more evident in the concentration of 500 μg/ml, which had a 24% increase in the fibroblast population. Conclusions: The ethanolic extract from A. angustifolia obtained by MAE, there was no evidence of cytotoxicity. It was able to be considered as a biocompatible material. Rather, it was shown to promote fibroblast proliferation.
KW - Cytotoxicity
KW - Human fibroblasts
KW - Microwave-assisted extraction
KW - β-sitosterol β-D-glucoside
UR - http://www.scopus.com/inward/record.url?scp=85119854207&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-82064-0_9
DO - 10.1007/978-3-030-82064-0_9
M3 - Contribución a la conferencia
AN - SCOPUS:85119854207
SN - 9783030820633
T3 - Lecture Notes in Networks and Systems
SP - 107
EP - 116
BT - Recent Trends in Sustainable Engineering - Proceedings of the 2nd International Conference on Applied Science and Advanced Technology
A2 - Flores Rodríguez, Karen Lizbeth
A2 - Ramos Alvarado, Raymundo
A2 - Barati, Masoud
A2 - Segovia Tagle, Veronica
A2 - Velázquez González, Roberto Sostrand
PB - Springer Science and Business Media Deutschland GmbH
T2 - 2nd International Conference on Applied Science and Advanced Technology, ICASAT 2021
Y2 - 2 June 2021 through 3 June 2021
ER -
