TY - JOUR
T1 - Antioxidant and anti-proliferative activity of free, conjugates and bound phenolic compounds from tomato and industrial tomato by-product
AU - Perea-Domínguez, Xiomara Patricia
AU - Mendoza-Espinoza, José Alberto
AU - Solano González, Jesús Martin
AU - Leyva-Morales, José Belisario
AU - Espinosa-Alonso, Laura Gabriela
AU - Valdez-Morales, Maribel
N1 - Publisher Copyright:
© 2021, Association of Food Scientists & Technologists (India).
PY - 2022/6
Y1 - 2022/6
N2 - The aim of this study was to evaluate the antioxidant and anti-proliferative activity of different fractions of phenolic compounds from tomato and tomato by-product. Soluble free phenolics and bound phenolics (BP) fractions from saladette tomato and industrial tomato by-product as well as, conjugated acid-hydrolysable phenolics (AHP) and alkaline-hydrolysable phenolics (AKHP) from saladette tomato, grape tomato and industrial tomato by-product, were tested. The scavenging effects of phenolic fractions on superoxide anion were determined using the PMS-NADH-NBT system. The myoglobin protection ratios of samples against hydroxyl radical were evaluated and the anti-proliferative effects in the MCF-7 breast cancer cell line were determined. AHP fraction from tomato by-product and AKHP fraction from grape and saladette tomato showed the highest inhibitory capacity of the superoxide anion (p ≤ 0.05). All samples had a myoglobin protection ratio from 30 to 50%. Regarding to cytotoxicity assays, all phenolic fractions from tomato by-product, as well as, BP, AHP, AKHP from saladette tomato and AHP from grape tomato showed important anti-proliferative activity against MCF-7 breast cancer cell line (IC50 < 20 μg/mL). From the results, we can conclude that tomato and tomato by-product are a good source of natural compound with important antioxidant and anti-proliferative activity.
AB - The aim of this study was to evaluate the antioxidant and anti-proliferative activity of different fractions of phenolic compounds from tomato and tomato by-product. Soluble free phenolics and bound phenolics (BP) fractions from saladette tomato and industrial tomato by-product as well as, conjugated acid-hydrolysable phenolics (AHP) and alkaline-hydrolysable phenolics (AKHP) from saladette tomato, grape tomato and industrial tomato by-product, were tested. The scavenging effects of phenolic fractions on superoxide anion were determined using the PMS-NADH-NBT system. The myoglobin protection ratios of samples against hydroxyl radical were evaluated and the anti-proliferative effects in the MCF-7 breast cancer cell line were determined. AHP fraction from tomato by-product and AKHP fraction from grape and saladette tomato showed the highest inhibitory capacity of the superoxide anion (p ≤ 0.05). All samples had a myoglobin protection ratio from 30 to 50%. Regarding to cytotoxicity assays, all phenolic fractions from tomato by-product, as well as, BP, AHP, AKHP from saladette tomato and AHP from grape tomato showed important anti-proliferative activity against MCF-7 breast cancer cell line (IC50 < 20 μg/mL). From the results, we can conclude that tomato and tomato by-product are a good source of natural compound with important antioxidant and anti-proliferative activity.
KW - Antioxidant capacity
KW - MCF-7 cell line
KW - Myoglobin protection
KW - Phenolic compounds
KW - Superoxide anion
KW - Tomato by-product
UR - http://www.scopus.com/inward/record.url?scp=85118505000&partnerID=8YFLogxK
U2 - 10.1007/s13197-021-05252-9
DO - 10.1007/s13197-021-05252-9
M3 - Artículo
C2 - 35602438
AN - SCOPUS:85118505000
SN - 0022-1155
VL - 59
SP - 2361
EP - 2369
JO - Journal of Food Science and Technology
JF - Journal of Food Science and Technology
IS - 6
ER -
