Plant regeneration of three Opuntia genotypes used as human food

Pedro A. García-Saucedo; Maribel Valdez-Morales; María Elena Valverde; Andrés Cruz-Hernández; Octavio Paredes-López

doi:10.1007/s11240-004-9158-0

Plant regeneration of three Opuntia genotypes used as human food

Pedro A. García-Saucedo, Maribel Valdez-Morales, María Elena Valverde, Andrés Cruz-Hernández, Octavio Paredes-López

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA₃. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	Plant Cell, Tissue and Organ Culture
Volume	80
Issue number	2
DOIs	https://doi.org/10.1007/s11240-004-9158-0
State	Published - Feb 2005
Externally published	Yes

Keywords

Acclimatization
Axillary buds
Cactaceae
Edible cladode
Micropropagation
Shoot induction

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10.1007/s11240-004-9158-0

Cite this

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title = "Plant regeneration of three Opuntia genotypes used as human food",

abstract = "In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.",

keywords = "Acclimatization, Axillary buds, Cactaceae, Edible cladode, Micropropagation, Shoot induction",

author = "Garc{\'i}a-Saucedo, {Pedro A.} and Maribel Valdez-Morales and Valverde, {Mar{\'i}a Elena} and Andr{\'e}s Cruz-Hern{\'a}ndez and Octavio Paredes-L{\'o}pez",

note = "Funding Information: This study was supported by CONCyTEG and CONACyT. MVM is affiliated to U. of Quer{\'e}taro. We thank Dr C. Mondrag{\'o}n-Jacobo, INIFAP, for nopal samples and Dr F. Guevara-Lara for reviewing the manuscript.",

year = "2005",

month = feb,

doi = "10.1007/s11240-004-9158-0",

language = "Ingl{\'e}s",

volume = "80",

pages = "215--219",

journal = "Plant Cell, Tissue and Organ Culture",

issn = "0167-6857",

number = "2",

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TY - JOUR

T1 - Plant regeneration of three Opuntia genotypes used as human food

AU - García-Saucedo, Pedro A.

AU - Valdez-Morales, Maribel

AU - Valverde, María Elena

AU - Cruz-Hernández, Andrés

AU - Paredes-López, Octavio

N1 - Funding Information: This study was supported by CONCyTEG and CONACyT. MVM is affiliated to U. of Querétaro. We thank Dr C. Mondragón-Jacobo, INIFAP, for nopal samples and Dr F. Guevara-Lara for reviewing the manuscript.

PY - 2005/2

Y1 - 2005/2

N2 - In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.

AB - In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.

KW - Acclimatization

KW - Axillary buds

KW - Cactaceae

KW - Edible cladode

KW - Micropropagation

KW - Shoot induction

UR - http://www.scopus.com/inward/record.url?scp=13244279619&partnerID=8YFLogxK

U2 - 10.1007/s11240-004-9158-0

DO - 10.1007/s11240-004-9158-0

M3 - Artículo

SN - 0167-6857

VL - 80

SP - 215

EP - 219

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 2

ER -

Plant regeneration of three Opuntia genotypes used as human food

Abstract

Keywords

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