TY - JOUR
T1 - Plant regeneration of three Opuntia genotypes used as human food
AU - García-Saucedo, Pedro A.
AU - Valdez-Morales, Maribel
AU - Valverde, María Elena
AU - Cruz-Hernández, Andrés
AU - Paredes-López, Octavio
N1 - Funding Information:
This study was supported by CONCyTEG and CONACyT. MVM is affiliated to U. of Querétaro. We thank Dr C. Mondragón-Jacobo, INIFAP, for nopal samples and Dr F. Guevara-Lara for reviewing the manuscript.
PY - 2005/2
Y1 - 2005/2
N2 - In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.
AB - In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 μM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.
KW - Acclimatization
KW - Axillary buds
KW - Cactaceae
KW - Edible cladode
KW - Micropropagation
KW - Shoot induction
UR - http://www.scopus.com/inward/record.url?scp=13244279619&partnerID=8YFLogxK
U2 - 10.1007/s11240-004-9158-0
DO - 10.1007/s11240-004-9158-0
M3 - Artículo
SN - 0167-6857
VL - 80
SP - 215
EP - 219
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 2
ER -