Particle bombardment, a method for gene transfer in marigold

P. E. Vanegas; M. Valdez-Morales; M. E. Valverde; A. Cruz-Hernández; O. Paredes-López

doi:10.1007/s11240-005-9030-x

Particle bombardment, a method for gene transfer in marigold

P. E. Vanegas, M. Valdez-Morales, M. E. Valverde, A. Cruz-Hernández, O. Paredes-López

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l^-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

Original language	English
Pages (from-to)	359-363
Number of pages	5
Journal	Plant Cell, Tissue and Organ Culture
Volume	84
Issue number	3
DOIs	https://doi.org/10.1007/s11240-005-9030-x
State	Published - Mar 2006
Externally published	Yes

Keywords

Biolistics
Genetic transformation
Organogenesis
Stable expression
Tagetes erecta

Access to Document

10.1007/s11240-005-9030-x

Cite this

@article{c06e571da0014d069b6908b14846bc81,

title = "Particle bombardment, a method for gene transfer in marigold",

abstract = "Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.",

keywords = "Biolistics, Genetic transformation, Organogenesis, Stable expression, Tagetes erecta",

author = "Vanegas, {P. E.} and M. Valdez-Morales and Valverde, {M. E.} and A. Cruz-Hern{\'a}ndez and O. Paredes-L{\'o}pez",

year = "2006",

month = mar,

doi = "10.1007/s11240-005-9030-x",

language = "Ingl{\'e}s",

volume = "84",

pages = "359--363",

journal = "Plant Cell, Tissue and Organ Culture",

issn = "0167-6857",

number = "3",

}

TY - JOUR

T1 - Particle bombardment, a method for gene transfer in marigold

AU - Vanegas, P. E.

AU - Valdez-Morales, M.

AU - Valverde, M. E.

AU - Cruz-Hernández, A.

AU - Paredes-López, O.

PY - 2006/3

Y1 - 2006/3

N2 - Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

AB - Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

KW - Biolistics

KW - Genetic transformation

KW - Organogenesis

KW - Stable expression

KW - Tagetes erecta

UR - http://www.scopus.com/inward/record.url?scp=33646470484&partnerID=8YFLogxK

U2 - 10.1007/s11240-005-9030-x

DO - 10.1007/s11240-005-9030-x

M3 - Artículo

SN - 0167-6857

VL - 84

SP - 359

EP - 363

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 3

ER -

Particle bombardment, a method for gene transfer in marigold

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this