Particle bombardment, a method for gene transfer in marigold

P. E. Vanegas; M. Valdez-Morales; M. E. Valverde; A. Cruz-Hernández; O. Paredes-López

doi:10.1007/s11240-005-9030-x

Particle bombardment, a method for gene transfer in marigold

P. E. Vanegas, M. Valdez-Morales, M. E. Valverde, A. Cruz-Hernández, O. Paredes-López

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12 Citas (Scopus)

Resumen

Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l^-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

Idioma original	Inglés
Páginas (desde-hasta)	359-363
Número de páginas	5
Publicación	Plant Cell, Tissue and Organ Culture
Volumen	84
N.º	3
DOI	https://doi.org/10.1007/s11240-005-9030-x
Estado	Publicada - mar. 2006
Publicado de forma externa	Sí

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title = "Particle bombardment, a method for gene transfer in marigold",

abstract = "Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.",

keywords = "Biolistics, Genetic transformation, Organogenesis, Stable expression, Tagetes erecta",

author = "Vanegas, {P. E.} and M. Valdez-Morales and Valverde, {M. E.} and A. Cruz-Hern{\'a}ndez and O. Paredes-L{\'o}pez",

year = "2006",

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doi = "10.1007/s11240-005-9030-x",

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T1 - Particle bombardment, a method for gene transfer in marigold

AU - Vanegas, P. E.

AU - Valdez-Morales, M.

AU - Valverde, M. E.

AU - Cruz-Hernández, A.

AU - Paredes-López, O.

PY - 2006/3

Y1 - 2006/3

N2 - Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

AB - Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l-1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

KW - Biolistics

KW - Genetic transformation

KW - Organogenesis

KW - Stable expression

KW - Tagetes erecta

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U2 - 10.1007/s11240-005-9030-x

DO - 10.1007/s11240-005-9030-x

M3 - Artículo

SN - 0167-6857

VL - 84

SP - 359

EP - 363

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 3

ER -

Particle bombardment, a method for gene transfer in marigold

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