Optimization of the conditions for the elaboration of chitosan nanoparticles charged with alpha lipoic acid, ascorbic acid and Alpha-Tocopherol

Advances in nano materials with antioxidant activity are considered as an emerging subject in food and pharmaceutical industries. The objective of the present work was to propose and optimize the necessary conditions for the formation of chitosan nanoparticles containing α-Lipoic acid, α-Tocopherol and ascorbic acid, as well as to determine the physicochemical and morphological properties of such particles. The determination of antioxidant activity was performed by the DPPH, FRAP and ABTS for the antioxidants mixture design in such a way that the mixture presenting the highest antioxidant activity was used in the preparation of nanoparticles that were analysed in terms of Z Potential, size and distribution, and observed in terms TEM. The design of ternary mixtures of ascorbic acid/α-Lipoic acid/α-Tocopherol showed the higher antioxidant activity with DPPH technique with the proportions: 250 mg/667 mg/183 mg respectively. Z Potential showed higher values at low pH and TPP and Tween 80 positively aαected in this parameter. Size and distribution of nanoparticles, the smaller size was obtained using TPP and Tween 80 with and without the presence of the antioxidant mixture, between 52 to 260 nm.