Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves

Gonzalo G. Lucho-Constantino, Fabiola Zaragoza-Martínez, Teresa Ponce-Noyola, Carlos M. Cerda-García-Rojas, Gabriela Trejo-Tapia, Fernando Esparza-García, Ana C. Ramos-Valdivia

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Abstract

© 2017, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.
Original languageAmerican English
JournalActa Physiologiae Plantarum
DOIs
StatePublished - 1 Aug 2017

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Jatropha
Anthocyanins
Jatropha curcas
antioxidants
jasmonic acid
Flavonoids
leaves
anthocyanins
catalase
flavonoids
Antioxidants
acids
Redox cells
plant physiology
phenylalanine
Ascorbate Peroxidases
metabolites
ascorbate peroxidase
ammonia
Catalase

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Lucho-Constantino, G. G., Zaragoza-Martínez, F., Ponce-Noyola, T., Cerda-García-Rojas, C. M., Trejo-Tapia, G., Esparza-García, F., & Ramos-Valdivia, A. C. (2017). Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves. Acta Physiologiae Plantarum. https://doi.org/10.1007/s11738-017-2461-2
Lucho-Constantino, Gonzalo G. ; Zaragoza-Martínez, Fabiola ; Ponce-Noyola, Teresa ; Cerda-García-Rojas, Carlos M. ; Trejo-Tapia, Gabriela ; Esparza-García, Fernando ; Ramos-Valdivia, Ana C. / Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves. In: Acta Physiologiae Plantarum. 2017.
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title = "Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves",
abstract = "{\circledC} 2017, Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w. Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58{\%} (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6{\%} increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6{\%} decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.",
author = "Lucho-Constantino, {Gonzalo G.} and Fabiola Zaragoza-Mart{\'i}nez and Teresa Ponce-Noyola and Cerda-Garc{\'i}a-Rojas, {Carlos M.} and Gabriela Trejo-Tapia and Fernando Esparza-Garc{\'i}a and Ramos-Valdivia, {Ana C.}",
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journal = "Acta Physiologiae Plantarum",
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Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves. / Lucho-Constantino, Gonzalo G.; Zaragoza-Martínez, Fabiola; Ponce-Noyola, Teresa; Cerda-García-Rojas, Carlos M.; Trejo-Tapia, Gabriela; Esparza-García, Fernando; Ramos-Valdivia, Ana C.

In: Acta Physiologiae Plantarum, 01.08.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves

AU - Lucho-Constantino, Gonzalo G.

AU - Zaragoza-Martínez, Fabiola

AU - Ponce-Noyola, Teresa

AU - Cerda-García-Rojas, Carlos M.

AU - Trejo-Tapia, Gabriela

AU - Esparza-García, Fernando

AU - Ramos-Valdivia, Ana C.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - © 2017, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.

AB - © 2017, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.

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