TY - JOUR
T1 - Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves
AU - Lucho-Constantino, Gonzalo G.
AU - Zaragoza-Martínez, Fabiola
AU - Ponce-Noyola, Teresa
AU - Cerda-García-Rojas, Carlos M.
AU - Trejo-Tapia, Gabriela
AU - Esparza-García, Fernando
AU - Ramos-Valdivia, Ana C.
N1 - Publisher Copyright:
© 2017, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.
AB - Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.
KW - Antioxidant responses
KW - Flavonoids
KW - Jasmonic acid
KW - Jatropha curcas
KW - Phenylalanine ammonia lyase
UR - http://www.scopus.com/inward/record.url?scp=85024122017&partnerID=8YFLogxK
U2 - 10.1007/s11738-017-2461-2
DO - 10.1007/s11738-017-2461-2
M3 - Artículo
SN - 0137-5881
VL - 39
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 8
M1 - 165
ER -