Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

A. N. Hernández-Lauzardo; S. Bautista-Baños; M. G. Velázquez-del Valle; M. G. Méndez-Montealvo; M. M. Sánchez-Rivera; L. A. Bello-Pérez

doi:10.1016/j.carbpol.2007.12.020

Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

A. N. Hernández-Lauzardo, S. Bautista-Baños, M. G. Velázquez-del Valle, M. G. Méndez-Montealvo, M. M. Sánchez-Rivera, L. A. Bello-Pérez

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Research output: Contribution to journal › Article › peer-review

175 Scopus citations

Abstract

Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.

Original language	English
Pages (from-to)	541-547
Number of pages	7
Journal	Carbohydrate Polymers
Volume	73
Issue number	4
DOIs	https://doi.org/10.1016/j.carbpol.2007.12.020
State	Published - 5 Sep 2008

Keywords

Antifungal activity
Chitosan
Image analysis
Spore ornamentations

Access to Document

10.1016/j.carbpol.2007.12.020

Cite this

Hernández-Lauzardo, A. N., Bautista-Baños, S., Velázquez-del Valle, M. G., Méndez-Montealvo, M. G., Sánchez-Rivera, M. M., & Bello-Pérez, L. A. (2008). Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. Carbohydrate Polymers, 73(4), 541-547. https://doi.org/10.1016/j.carbpol.2007.12.020

@article{6abf4b7e046d4f8092346aea6af50742,

title = "Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill",

abstract = "Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.",

keywords = "Antifungal activity, Chitosan, Image analysis, Spore ornamentations",

author = "Hern{\'a}ndez-Lauzardo, {A. N.} and S. Bautista-Ba{\~n}os and {Vel{\'a}zquez-del Valle}, {M. G.} and M{\'e}ndez-Montealvo, {M. G.} and S{\'a}nchez-Rivera, {M. M.} and Bello-P{\'e}rez, {L. A.}",

note = "Funding Information: We are grateful to the National Polytechnic Institute for financial support and Eng. Patricia Castillo for technical work in SEM and TEM studies.",

year = "2008",

month = sep,

day = "5",

doi = "10.1016/j.carbpol.2007.12.020",

language = "Ingl{\'e}s",

volume = "73",

pages = "541--547",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

number = "4",

}

Hernández-Lauzardo, AN, Bautista-Baños, S , Velázquez-del Valle, MG , Méndez-Montealvo, MG , Sánchez-Rivera, MM & Bello-Pérez, LA 2008, 'Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill', Carbohydrate Polymers, vol. 73, no. 4, pp. 541-547. https://doi.org/10.1016/j.carbpol.2007.12.020

TY - JOUR

T1 - Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

AU - Hernández-Lauzardo, A. N.

AU - Bautista-Baños, S.

AU - Velázquez-del Valle, M. G.

AU - Méndez-Montealvo, M. G.

AU - Sánchez-Rivera, M. M.

AU - Bello-Pérez, L. A.

N1 - Funding Information: We are grateful to the National Polytechnic Institute for financial support and Eng. Patricia Castillo for technical work in SEM and TEM studies.

PY - 2008/9/5

Y1 - 2008/9/5

N2 - Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.

AB - Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.

KW - Antifungal activity

KW - Chitosan

KW - Image analysis

KW - Spore ornamentations

UR - http://www.scopus.com/inward/record.url?scp=43349087931&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2007.12.020

DO - 10.1016/j.carbpol.2007.12.020

M3 - Artículo

C2 - 26048219

SN - 0144-8617

VL - 73

SP - 541

EP - 547

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 4

ER -

Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this