Active biopolymeric films inoculated with bdellovibrio bacteriovorus, a predatory bacterium

Christian Mariel Sáenz-Santos, Omotayo Opemipo Oyedara, Yunia Verónica García-Tejeda, Claudia A. Romero-Bastida, Esperanza Milagros García-Oropesa, Eduardo Villalobo, Mario A. Rodríguez-Pérez

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the film formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric films. FTIR spectroscopy provided information about the structural composition of the films. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli.

Original languageEnglish
Article number605
Issue number5
StatePublished - May 2021


  • Active film
  • Bdellovibrio entrapment
  • Collagen
  • Sodium alginate
  • Starch


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