TY - JOUR
T1 - Active biopolymeric films inoculated with bdellovibrio bacteriovorus, a predatory bacterium
AU - Sáenz-Santos, Christian Mariel
AU - Opemipo Oyedara, Omotayo
AU - García-Tejeda, Yunia Verónica
AU - Romero-Bastida, Claudia A.
AU - García-Oropesa, Esperanza Milagros
AU - Villalobo, Eduardo
AU - Rodríguez-Pérez, Mario A.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/5
Y1 - 2021/5
N2 - The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the film formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric films. FTIR spectroscopy provided information about the structural composition of the films. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli.
AB - The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the film formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric films. FTIR spectroscopy provided information about the structural composition of the films. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli.
KW - Active film
KW - Bdellovibrio entrapment
KW - Collagen
KW - Sodium alginate
KW - Starch
UR - http://www.scopus.com/inward/record.url?scp=85107007362&partnerID=8YFLogxK
U2 - 10.3390/coatings11050605
DO - 10.3390/coatings11050605
M3 - Artículo
AN - SCOPUS:85107007362
SN - 2079-6412
VL - 11
JO - Coatings
JF - Coatings
IS - 5
M1 - 605
ER -