Synthesis and evaluation of antibacterial activity of danazol-succinate-lisozyme conjugate on staphylococcus Aureus

In this work, danazol-succinate-lisozyme conjugate was synthesized (5); the route involved the preparation of danazol succinate (3) by esterification of danazol (1) with succinic anhydride (2) followed by the reaction of 3 with lisozyme (4) in order to obtain 5. The structure of 3 was confirmed by spectroscopy and spectrometry data and 5 was characterized by spectroscopy ultraviolet andelectrophoresis analysis. The antibacterial activity of compound 3 and 5 was evaluated in vitro on Staphylococcus aureus, using dilution method and the minimum inhibitory concentration. The results showed that 11 residues of steroid-derivative (3) were coupled on a lisozyme molecule (4), obtaining 5.23 × 10-5 mmol of danazol-succinate per milligram of 5. Electrophoresis analysis showed a band of ≅ 16 Kda for 5. Other experimental data indicate that bacterial growth of Staphylococcus aureus in presence of 3 and 5 it was inhibited following a behavior of the type dose-dependent. In conclusion, experimental data suggest that antibacterial activity of danazol-succinate-lisozyme depend on interaction of protein with danazol-succinate, in order to interact with the cell surface, and perturb bacterial growth of Staphylococcus aureus.