Synthesis and evaluation of antibacterial activity of danazol-succinate-lisozyme conjugate on staphylococcus Aureus

V. L. Figueroa, C. F. Díaz, R. M. López, L. A. Camacho

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2 Citations (Scopus)

Abstract

In this work, danazol-succinate-lisozyme conjugate was synthesized (5); the route involved the preparation of danazol succinate (3) by esterification of danazol (1) with succinic anhydride (2) followed by the reaction of 3 with lisozyme (4) in order to obtain 5. The structure of 3 was confirmed by spectroscopy and spectrometry data and 5 was characterized by spectroscopy ultraviolet andelectrophoresis analysis. The antibacterial activity of compound 3 and 5 was evaluated in vitro on Staphylococcus aureus, using dilution method and the minimum inhibitory concentration. The results showed that 11 residues of steroid-derivative (3) were coupled on a lisozyme molecule (4), obtaining 5.23 × 10-5 mmol of danazol-succinate per milligram of 5. Electrophoresis analysis showed a band of ≅ 16 Kda for 5. Other experimental data indicate that bacterial growth of Staphylococcus aureus in presence of 3 and 5 it was inhibited following a behavior of the type dose-dependent. In conclusion, experimental data suggest that antibacterial activity of danazol-succinate-lisozyme depend on interaction of protein with danazol-succinate, in order to interact with the cell surface, and perturb bacterial growth of Staphylococcus aureus.
Original languageAmerican English
Pages (from-to)40-50
Number of pages34
JournalJournal of the Argentine Chemical Society
StatePublished - 1 Jul 2009

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Danazol
Succinic Acid
Esterification
Electrophoresis
Ultraviolet spectroscopy
Spectrometry
Dilution
Spectroscopy
Derivatives
Proteins
Molecules
Steroids

Cite this

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title = "Synthesis and evaluation of antibacterial activity of danazol-succinate-lisozyme conjugate on staphylococcus Aureus",
abstract = "In this work, danazol-succinate-lisozyme conjugate was synthesized (5); the route involved the preparation of danazol succinate (3) by esterification of danazol (1) with succinic anhydride (2) followed by the reaction of 3 with lisozyme (4) in order to obtain 5. The structure of 3 was confirmed by spectroscopy and spectrometry data and 5 was characterized by spectroscopy ultraviolet andelectrophoresis analysis. The antibacterial activity of compound 3 and 5 was evaluated in vitro on Staphylococcus aureus, using dilution method and the minimum inhibitory concentration. The results showed that 11 residues of steroid-derivative (3) were coupled on a lisozyme molecule (4), obtaining 5.23 × 10-5 mmol of danazol-succinate per milligram of 5. Electrophoresis analysis showed a band of ≅ 16 Kda for 5. Other experimental data indicate that bacterial growth of Staphylococcus aureus in presence of 3 and 5 it was inhibited following a behavior of the type dose-dependent. In conclusion, experimental data suggest that antibacterial activity of danazol-succinate-lisozyme depend on interaction of protein with danazol-succinate, in order to interact with the cell surface, and perturb bacterial growth of Staphylococcus aureus.",
author = "Figueroa, {V. L.} and D{\'i}az, {C. F.} and L{\'o}pez, {R. M.} and Camacho, {L. A.}",
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journal = "Journal of the Argentine Chemical Society",
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Synthesis and evaluation of antibacterial activity of danazol-succinate-lisozyme conjugate on staphylococcus Aureus. / Figueroa, V. L.; Díaz, C. F.; López, R. M.; Camacho, L. A.

In: Journal of the Argentine Chemical Society, 01.07.2009, p. 40-50.

Research output: Contribution to journalArticle

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