TY - JOUR
T1 - Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus
AU - Reyes-Ruiz, José M.
AU - Osuna-Ramos, Juan F.
AU - Cervantes-Salazar, Margot
AU - Lagunes Guillen, Anel E.
AU - Chávez-Munguía, Bibiana
AU - Salas-Benito, Juan S.
AU - Del Ángel, Rosa M.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/2
Y1 - 2018/2
N2 - Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.
AB - Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.
KW - Dengue
KW - Nucleus
KW - Strand-like structures
KW - Viral proteins
UR - http://www.scopus.com/inward/record.url?scp=85038953168&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2017.12.014
DO - 10.1016/j.virol.2017.12.014
M3 - Artículo
C2 - 29272748
SN - 0042-6822
VL - 515
SP - 74
EP - 80
JO - Virology
JF - Virology
ER -
