Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus

José M. Reyes-Ruiz; Juan F. Osuna-Ramos; Margot Cervantes-Salazar; Anel E. Lagunes Guillen; Bibiana Chávez-Munguía; Juan S. Salas-Benito; Rosa M. Del Ángel

doi:10.1016/j.virol.2017.12.014

Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus

José M. Reyes-Ruiz, Juan F. Osuna-Ramos, Margot Cervantes-Salazar, Anel E. Lagunes Guillen, Bibiana Chávez-Munguía, Juan S. Salas-Benito, Rosa M. Del Ángel

Escuela Nacional de Medicina y Homeopatía (ENMH)

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.

Original language	English
Pages (from-to)	74-80
Number of pages	7
Journal	Virology
Volume	515
DOIs	https://doi.org/10.1016/j.virol.2017.12.014
State	Published - Feb 2018

Keywords

Dengue
Nucleus
Strand-like structures
Viral proteins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2017.12.014

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@article{51c4f6272b184b4fad1f29ded3a7518f,

title = "Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus",

abstract = "Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.",

keywords = "Dengue, Nucleus, Strand-like structures, Viral proteins",

author = "Reyes-Ruiz, {Jos{\'e} M.} and Osuna-Ramos, {Juan F.} and Margot Cervantes-Salazar and {Lagunes Guillen}, {Anel E.} and Bibiana Ch{\'a}vez-Mungu{\'i}a and Salas-Benito, {Juan S.} and {Del {\'A}ngel}, {Rosa M.}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2018",

month = feb,

doi = "10.1016/j.virol.2017.12.014",

language = "Ingl{\'e}s",

volume = "515",

pages = "74--80",

journal = "Virology",

issn = "0042-6822",

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TY - JOUR

T1 - Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus

AU - Reyes-Ruiz, José M.

AU - Osuna-Ramos, Juan F.

AU - Cervantes-Salazar, Margot

AU - Lagunes Guillen, Anel E.

AU - Chávez-Munguía, Bibiana

AU - Salas-Benito, Juan S.

AU - Del Ángel, Rosa M.

PY - 2018/2

Y1 - 2018/2

N2 - Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.

AB - Dengue virus (DENV) is an arbovirus, which replicates in the endoplasmic reticulum. Although replicative cycle takes place in the cytoplasm, some viral proteins such as NS5 and C are translocated to the nucleus during infection in mosquitoes and mammalian cells. To localized viral proteins in DENV-infected C6/36 cells, an immunofluorescence (IF) and immunoelectron microscopy (IEM) analysis were performed. Our results indicated that C, NS1, NS3 and NS5 proteins were found in the nucleus of DENV-infected C6/36 cells. Additionally, complex structures named strand-like structures (Ss) were observed in the nucleus of infected cells. Interestingly, the NS5 protein was located in these structures. Ss were absent in mock-infected cells, suggesting that DENV induces their formation in the nucleus of infected mosquito cells.

KW - Dengue

KW - Nucleus

KW - Strand-like structures

KW - Viral proteins

UR - http://www.scopus.com/inward/record.url?scp=85038953168&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2017.12.014

DO - 10.1016/j.virol.2017.12.014

M3 - Artículo

C2 - 29272748

SN - 0042-6822

VL - 515

SP - 74

EP - 80

JO - Virology

JF - Virology

ER -

Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus

Abstract

Keywords

UN SDGs

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