Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

Nadia Ramírez; Damaris Lorenzo; Daniel Palenzuela; Lissett Herrera; Marta Ayala; Alejandro Fuentes; Marlene Pérez; Jorge Gavilondo; Pedro Oramas

doi:10.1023/A:1005605214703

Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

Nadia Ramírez, Damaris Lorenzo, Daniel Palenzuela, Lissett Herrera, Marta Ayala, Alejandro Fuentes, Marlene Pérez, Jorge Gavilondo, Pedro Oramas

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15 Citas (Scopus)

Resumen

An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l^-1) and in cells (5 mg kg^-1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.

Idioma original	Inglés
Páginas (desde-hasta)	1233-1236
Número de páginas	4
Publicación	Biotechnology Letters
Volumen	22
N.º	15
DOI	https://doi.org/10.1023/A:1005605214703
Estado	Publicada - 2000
Publicado de forma externa	Sí

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title = "Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures",

abstract = "An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l-1) and in cells (5 mg kg-1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.",

keywords = "Cell culture, Hepatitis B antigen, Recombinant antibodies, Transgenic plants",

author = "Nadia Ram{\'i}rez and Damaris Lorenzo and Daniel Palenzuela and Lissett Herrera and Marta Ayala and Alejandro Fuentes and Marlene P{\'e}rez and Jorge Gavilondo and Pedro Oramas",

year = "2000",

doi = "10.1023/A:1005605214703",

language = "Ingl{\'e}s",

volume = "22",

pages = "1233--1236",

journal = "Biotechnology Letters",

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TY - JOUR

T1 - Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

AU - Ramírez, Nadia

AU - Lorenzo, Damaris

AU - Palenzuela, Daniel

AU - Herrera, Lissett

AU - Ayala, Marta

AU - Fuentes, Alejandro

AU - Pérez, Marlene

AU - Gavilondo, Jorge

AU - Oramas, Pedro

PY - 2000

Y1 - 2000

N2 - An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l-1) and in cells (5 mg kg-1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.

AB - An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l-1) and in cells (5 mg kg-1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.

KW - Cell culture

KW - Hepatitis B antigen

KW - Recombinant antibodies

KW - Transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=0033823456&partnerID=8YFLogxK

U2 - 10.1023/A:1005605214703

DO - 10.1023/A:1005605214703

M3 - Artículo

AN - SCOPUS:0033823456

SN - 0141-5492

VL - 22

SP - 1233

EP - 1236

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 15

ER -

Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

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