Abstract
An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l-1) and in cells (5 mg kg-1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.
Original language | English |
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Pages (from-to) | 1233-1236 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 22 |
Issue number | 15 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Cell culture
- Hepatitis B antigen
- Recombinant antibodies
- Transgenic plants