TY - JOUR
T1 - Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma
AU - Flores Pérez, Carmen
AU - Juárez Olguín, Hugo
AU - Flores Pérez, Janett
AU - Toledo López, Alejandra
AU - Lares Asseff, Ismael
AU - Alvarez García, Carlos
PY - 2003/9/25
Y1 - 2003/9/25
N2 - The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C18 column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.
AB - The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C18 column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.
KW - Ranitidine
UR - http://www.scopus.com/inward/record.url?scp=0142042999&partnerID=8YFLogxK
U2 - 10.1016/S1570-0232(03)00474-4
DO - 10.1016/S1570-0232(03)00474-4
M3 - Artículo
C2 - 12957178
AN - SCOPUS:0142042999
SN - 1570-0232
VL - 795
SP - 141
EP - 144
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -