Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma

Carmen Flores Pérez; Hugo Juárez Olguín; Janett Flores Pérez; Alejandra Toledo López; Ismael Lares Asseff; Carlos Alvarez García

doi:10.1016/S1570-0232(03)00474-4

Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma

Carmen Flores Pérez, Hugo Juárez Olguín, Janett Flores Pérez, Alejandra Toledo López, Ismael Lares Asseff, Carlos Alvarez García

Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Durango

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12 Scopus citations

Abstract

The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C₁₈ column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml^-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml^-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	795
Issue number	1
DOIs	https://doi.org/10.1016/S1570-0232(03)00474-4
State	Published - 25 Sep 2003

Keywords

Ranitidine

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10.1016/S1570-0232(03)00474-4

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Flores Pérez, C., Juárez Olguín, H., Flores Pérez, J., Toledo López, A., Lares Asseff, I., & Alvarez García, C. (2003). Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 795(1), 141-144. https://doi.org/10.1016/S1570-0232(03)00474-4

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abstract = "The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C18 column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.",

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Flores Pérez, C, Juárez Olguín, H, Flores Pérez, J, Toledo López, A, Lares Asseff, I & Alvarez García, C 2003, 'Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 795, no. 1, pp. 141-144. https://doi.org/10.1016/S1570-0232(03)00474-4

Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma. / Flores Pérez, Carmen; Juárez Olguín, Hugo; Flores Pérez, Janett et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 795, No. 1, 25.09.2003, p. 141-144.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma

AU - Flores Pérez, Carmen

AU - Juárez Olguín, Hugo

AU - Flores Pérez, Janett

AU - Toledo López, Alejandra

AU - Lares Asseff, Ismael

AU - Alvarez García, Carlos

PY - 2003/9/25

Y1 - 2003/9/25

N2 - The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C18 column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.

AB - The aim of the present study was to develop a simple method to measure ranitidine, using 100 μl of plasma, by high-performance liquid chromatography with a Symmetry C18 column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml-1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml-1, respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.

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JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1

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Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma

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