TY - JOUR
T1 - Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high-performance liquid chromatography and its application in a pharmacokinetic study
AU - Aguilar-Carrasco, José Carlos
AU - Hernández-Pineda, Jessica
AU - Jiménez-Andrade, Juan Miguel
AU - Flores-Murrieta, Francisco Javier
AU - Carrasco-Portugal, Miriam del Carmen
AU - López-Canales, Jorge Skiold
N1 - Publisher Copyright:
© 2014 John Wiley & Sons, Ltd.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - A rapid, sensitive and simple high-performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r≥0.9992) over the concentration range 0.1-12 μg/mL. The average absolute recovery was 93.59%. The intra- and inter-day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost-effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample.
AB - A rapid, sensitive and simple high-performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r≥0.9992) over the concentration range 0.1-12 μg/mL. The average absolute recovery was 93.59%. The intra- and inter-day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost-effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample.
KW - HPLC
KW - Human plasma
KW - Levofloxacin
KW - Microsamples
KW - Pharmacokinetics
UR - http://www.scopus.com/inward/record.url?scp=84920938933&partnerID=8YFLogxK
U2 - 10.1002/bmc.3278
DO - 10.1002/bmc.3278
M3 - Artículo
SN - 0269-3879
VL - 29
SP - 341
EP - 345
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 3
ER -