Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high-performance liquid chromatography and its application in a pharmacokinetic study

José Carlos Aguilar-Carrasco, Jessica Hernández-Pineda, Juan Miguel Jiménez-Andrade, Francisco Javier Flores-Murrieta, Miriam del Carmen Carrasco-Portugal, Jorge Skiold López-Canales

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

© 2014 John Wiley & Sons, Ltd. A rapid, sensitive and simple high-performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r≥0.9992) over the concentration range 0.1-12 μg/mL. The average absolute recovery was 93.59%. The intra- and inter-day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost-effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample.
Original languageAmerican English
Pages (from-to)341-345
Number of pages306
JournalBiomedical Chromatography
DOIs
StatePublished - 1 Jan 2015

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Plasma (human)
Levofloxacin
Pharmacokinetics
High performance liquid chromatography
High Pressure Liquid Chromatography
Cost effectiveness
Assays
Potassium
Blood Volume
Nuclear Family
Blood
Running
Cost-Benefit Analysis
Healthy Volunteers
Recovery
Liquids
Proteins

Cite this

Aguilar-Carrasco, José Carlos ; Hernández-Pineda, Jessica ; Jiménez-Andrade, Juan Miguel ; Flores-Murrieta, Francisco Javier ; Carrasco-Portugal, Miriam del Carmen ; López-Canales, Jorge Skiold. / Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high-performance liquid chromatography and its application in a pharmacokinetic study. In: Biomedical Chromatography. 2015 ; pp. 341-345.
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Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high-performance liquid chromatography and its application in a pharmacokinetic study. / Aguilar-Carrasco, José Carlos; Hernández-Pineda, Jessica; Jiménez-Andrade, Juan Miguel; Flores-Murrieta, Francisco Javier; Carrasco-Portugal, Miriam del Carmen; López-Canales, Jorge Skiold.

In: Biomedical Chromatography, 01.01.2015, p. 341-345.

Research output: Contribution to journalArticle

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AU - López-Canales, Jorge Skiold

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