Resumen
Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region - 950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.
Idioma original | Inglés |
---|---|
Páginas (desde-hasta) | 320-325 |
Número de páginas | 6 |
Publicación | Biochimica et Biophysica Acta - Gene Structure and Expression |
Volumen | 1442 |
N.º | 2-3 |
DOI | |
Estado | Publicada - 8 nov. 1998 |
Publicado de forma externa | Sí |