Promoter analysis of the acetate-inducible isocitrate lyase gene (acu- 3) from Neurospora crassa

Martha Bibbins; Peter J. Sheffield; Laurence D.S. Gainey; Tomoko Mizote; Ian F. Connerton

doi:10.1016/S0167-4781(98)00194-8

Promoter analysis of the acetate-inducible isocitrate lyase gene (acu- 3) from Neurospora crassa

Martha Bibbins, Peter J. Sheffield, Laurence D.S. Gainey, Tomoko Mizote, Ian F. Connerton

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Abstract

Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region - 950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.

Original language	English
Pages (from-to)	320-325
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1442
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(98)00194-8
State	Published - 8 Nov 1998
Externally published	Yes

Keywords

(Neurospora crassa)
Acetate metabolism
Gene regulation
Glyoxylate cycle
Isocitrate lyase

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10.1016/S0167-4781(98)00194-8

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title = "Promoter analysis of the acetate-inducible isocitrate lyase gene (acu- 3) from Neurospora crassa",

abstract = "Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region - 950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.",

keywords = "(Neurospora crassa), Acetate metabolism, Gene regulation, Glyoxylate cycle, Isocitrate lyase",

author = "Martha Bibbins and Sheffield, {Peter J.} and Gainey, {Laurence D.S.} and Tomoko Mizote and Connerton, {Ian F.}",

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TY - JOUR

T1 - Promoter analysis of the acetate-inducible isocitrate lyase gene (acu- 3) from Neurospora crassa

AU - Bibbins, Martha

AU - Sheffield, Peter J.

AU - Gainey, Laurence D.S.

AU - Mizote, Tomoko

AU - Connerton, Ian F.

PY - 1998/11/8

Y1 - 1998/11/8

N2 - Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region - 950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.

AB - Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region - 950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.

KW - (Neurospora crassa)

KW - Acetate metabolism

KW - Gene regulation

KW - Glyoxylate cycle

KW - Isocitrate lyase

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U2 - 10.1016/S0167-4781(98)00194-8

DO - 10.1016/S0167-4781(98)00194-8

M3 - Artículo

SN - 0167-4781

VL - 1442

SP - 320

EP - 325

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2-3

ER -

Promoter analysis of the acetate-inducible isocitrate lyase gene (acu- 3) from Neurospora crassa

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Keywords

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