TY - JOUR
T1 - Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin
AU - Elizalde-Cárdenas, Alejandro
AU - González-Torres, Maykel
AU - Leyva-Gómez, Gerardo
AU - Cortés, Hernán
AU - González-Mendoza, Oswaldo
AU - Pérez-Díaz, Mario Alberto
AU - Pineda, Carlos
AU - Ribas-Aparicio, Rosa María
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/10/1
Y1 - 2022/10/1
N2 - Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.
AB - Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.
KW - Cell proliferation
KW - Chitosan
KW - Copolymer
KW - N-hydroxyethyl acrylamide
KW - Skin
KW - γ-Radiation
UR - http://www.scopus.com/inward/record.url?scp=85133900329&partnerID=8YFLogxK
U2 - 10.1016/j.matlet.2022.132783
DO - 10.1016/j.matlet.2022.132783
M3 - Artículo
AN - SCOPUS:85133900329
SN - 0167-577X
VL - 324
JO - Materials Letters
JF - Materials Letters
M1 - 132783
ER -