Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin

Alejandro Elizalde-Cárdenas; Maykel González-Torres; Gerardo Leyva-Gómez; Hernán Cortés; Oswaldo González-Mendoza; Mario Alberto Pérez-Díaz; Carlos Pineda; Rosa María Ribas-Aparicio

doi:10.1016/j.matlet.2022.132783

Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin

Alejandro Elizalde-Cárdenas, Maykel González-Torres, Gerardo Leyva-Gómez, Hernán Cortés, Oswaldo González-Mendoza, Mario Alberto Pérez-Díaz, Carlos Pineda, Rosa María Ribas-Aparicio

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.

Original language	English
Article number	132783
Journal	Materials Letters
Volume	324
DOIs	https://doi.org/10.1016/j.matlet.2022.132783
State	Published - 1 Oct 2022

Keywords

Cell proliferation
Chitosan
Copolymer
N-hydroxyethyl acrylamide
Skin
γ-Radiation

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title = "Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin",

abstract = "Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.",

keywords = "Cell proliferation, Chitosan, Copolymer, N-hydroxyethyl acrylamide, Skin, γ-Radiation",

author = "Alejandro Elizalde-C{\'a}rdenas and Maykel Gonz{\'a}lez-Torres and Gerardo Leyva-G{\'o}mez and Hern{\'a}n Cort{\'e}s and Oswaldo Gonz{\'a}lez-Mendoza and P{\'e}rez-D{\'i}az, {Mario Alberto} and Carlos Pineda and Ribas-Aparicio, {Rosa Mar{\'i}a}",

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year = "2022",

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doi = "10.1016/j.matlet.2022.132783",

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T1 - Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin

AU - Elizalde-Cárdenas, Alejandro

AU - González-Torres, Maykel

AU - Leyva-Gómez, Gerardo

AU - Cortés, Hernán

AU - González-Mendoza, Oswaldo

AU - Pérez-Díaz, Mario Alberto

AU - Pineda, Carlos

AU - Ribas-Aparicio, Rosa María

PY - 2022/10/1

Y1 - 2022/10/1

N2 - Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.

AB - Amino-polysaccharides derivatives such as grafted chitosan (CS) have attracted increasing interest in biotechnology. The work aims to assess the novel CS-g-PHEAA as a model for in vitro-engineered skin. Gamma radiation-induced copolymerization has achieved N-hydroxyethyl acrylamide (HEAA) grafting onto CS. The functionalization of CS by the amino group has been confirmed through solid-state carbon-13 nuclear magnetic resonance. As evidenced by cell metabolic activity, viability, and proliferation results of human dermal fibroblast (HDF) co-cultivated with the biomaterials, the biological assays are independent of the choice of solvent. Also, the grafting degree, the HDF growth, and monolayer migration favored using CS-g-PHEAA prepared in bulk. Hence, it has potential as a future bioengineered skin substitute material.

KW - Cell proliferation

KW - Chitosan

KW - Copolymer

KW - N-hydroxyethyl acrylamide

KW - Skin

KW - γ-Radiation

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DO - 10.1016/j.matlet.2022.132783

M3 - Artículo

AN - SCOPUS:85133900329

SN - 0167-577X

VL - 324

JO - Materials Letters

JF - Materials Letters

M1 - 132783

ER -

Preparation of chitosan-graft N-hydroxyethyl acrylamide copolymers as an in vitro-engineered skin

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Keywords

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