TY - JOUR
T1 - Physiological and Biochemical Responses of Bicarbonate Supplementation on Biomass and Lipid Content of Green Algae Scenedesmus sp. BHU1 Isolated From Wastewater for Renewable Biofuel Feedstock
AU - Singh, Rahul Prasad
AU - Yadav, Priya
AU - Kumar, Ajay
AU - Hashem, Abeer
AU - Al-Arjani, Al Bandari Fahad
AU - Abd_Allah, Elsayed Fathi
AU - Rodríguez Dorantes, Angélica
AU - Gupta, Rajan Kumar
N1 - Publisher Copyright:
Copyright © 2022 Singh, Yadav, Kumar, Hashem, Al-Arjani, Abd_Allah, Rodríguez Dorantes and Gupta.
PY - 2022/3/29
Y1 - 2022/3/29
N2 - In the present study, different microalgae were isolated from wastewater environment and evaluated for higher growth and lipid accumulation. The growth adaptability of all the isolated microalgae were tested for carbon source with supplementation of sodium bicarbonate in BG-11 N+ medium. Further based on the uptake rate of sodium bicarbonate and growth behavior, microalgal strains were selected for biofuel feedstock. During the study, growth parameters of all the isolates were screened after supplementation with various carbon sources, in which strain Scenedesmus sp. BHU1 was found highly effective among all. The efficacy of Scenedesmus sp. BHU1 strain under different sodium bicarbonate (4–20 mM) concentration, in which higher growth 1.4 times greater than control was observed at the concentration 12 mM sodium bicarbonate. In addition, total chlorophyll content (Chl-a + Chl-b), chlorophyll fluorescence (Fv/Fm, Y(II), ETR max, and NPQmax), and biomass productivity were found to be 11.514 μg/ml, 0.673, 0.675, and 31.167 μmol electrons m−2 s−1, 1.399, 59.167 mg/L/day, respectively, at the 12 mM sodium bicarbonate. However, under optimum sodium bicarbonate supplementation, 56.920% carbohydrate and 34.693% lipid content were accumulated, which showed potential of sodium bicarbonate supplementation in renewable biofuel feedstock by using Scenedesmus sp. BHU1 strain.
AB - In the present study, different microalgae were isolated from wastewater environment and evaluated for higher growth and lipid accumulation. The growth adaptability of all the isolated microalgae were tested for carbon source with supplementation of sodium bicarbonate in BG-11 N+ medium. Further based on the uptake rate of sodium bicarbonate and growth behavior, microalgal strains were selected for biofuel feedstock. During the study, growth parameters of all the isolates were screened after supplementation with various carbon sources, in which strain Scenedesmus sp. BHU1 was found highly effective among all. The efficacy of Scenedesmus sp. BHU1 strain under different sodium bicarbonate (4–20 mM) concentration, in which higher growth 1.4 times greater than control was observed at the concentration 12 mM sodium bicarbonate. In addition, total chlorophyll content (Chl-a + Chl-b), chlorophyll fluorescence (Fv/Fm, Y(II), ETR max, and NPQmax), and biomass productivity were found to be 11.514 μg/ml, 0.673, 0.675, and 31.167 μmol electrons m−2 s−1, 1.399, 59.167 mg/L/day, respectively, at the 12 mM sodium bicarbonate. However, under optimum sodium bicarbonate supplementation, 56.920% carbohydrate and 34.693% lipid content were accumulated, which showed potential of sodium bicarbonate supplementation in renewable biofuel feedstock by using Scenedesmus sp. BHU1 strain.
KW - Scenedesmussp
KW - biofuel
KW - chlorophyll fluorescence
KW - molecular analysis
KW - scanning electron microscopy
KW - sodium bicarbonate
UR - http://www.scopus.com/inward/record.url?scp=85128753874&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2022.839800
DO - 10.3389/fmicb.2022.839800
M3 - Artículo
C2 - 35444634
AN - SCOPUS:85128753874
SN - 1664-302X
VL - 13
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 839800
ER -