TY - JOUR
T1 - Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance
AU - Ortega-Amaro, Maria A.
AU - Rodriguez-Hernandez, Aida A.
AU - Rodriguez-Kessler, Margarita
AU - Hernandez-Lucero, Elolsa
AU - Rosales-Mendoza, Sergio
AU - Ibanez-Salazar, Alejandro
AU - Delgado-Sanchez, Pablo
AU - Jimenez-Bremont, Juan F.
N1 - Publisher Copyright:
© 2015 Ortega-Amaro, Rodríguez-Hernández, Rodríguez-Kessler, Hernández-Lucero, Rosales-Mendoza, Ibáñez-Salazar, Delgado-Sánchez and Jiménez-Bremont. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
PY - 2015/1/20
Y1 - 2015/1/20
N2 - Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.
AB - Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.
KW - Arabidopsis thaliana
KW - Development
KW - Glycine-rich domain protein
KW - Indole-3-acetic acid
KW - Lactuca sativa
KW - Salt stress
UR - http://www.scopus.com/inward/record.url?scp=84922261206&partnerID=8YFLogxK
U2 - 10.3389/fpls.2014.00782
DO - 10.3389/fpls.2014.00782
M3 - Artículo
AN - SCOPUS:84922261206
SN - 1664-462X
VL - 5
SP - 1
EP - 16
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
IS - JAN
M1 - 782
ER -