Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

Maria A. Ortega-Amaro, Aida A. Rodriguez-Hernandez, Margarita Rodriguez-Kessler, Elolsa Hernandez-Lucero, Sergio Rosales-Mendoza, Alejandro Ibanez-Salazar, Pablo Delgado-Sanchez, Juan F. Jimenez-Bremont

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.

Original languageEnglish
Article number782
Pages (from-to)1-16
Number of pages16
JournalFrontiers in Plant Science
Volume5
Issue numberJAN
DOIs
StatePublished - 20 Jan 2015

Fingerprint

stress tolerance
Arabidopsis
plant growth
proteins
mutants
lettuce
auxins
flowering
genes
indole acetic acid
salt stress
stress response
growth and development
genetically modified organisms
RNA
fungi
genetic markers
bacteria
organisms

Keywords

  • Arabidopsis thaliana
  • Development
  • Glycine-rich domain protein
  • Indole-3-acetic acid
  • Lactuca sativa
  • Salt stress

Cite this

Ortega-Amaro, M. A., Rodriguez-Hernandez, A. A., Rodriguez-Kessler, M., Hernandez-Lucero, E., Rosales-Mendoza, S., Ibanez-Salazar, A., ... Jimenez-Bremont, J. F. (2015). Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance. Frontiers in Plant Science, 5(JAN), 1-16. [782]. https://doi.org/10.3389/fpls.2014.00782
Ortega-Amaro, Maria A. ; Rodriguez-Hernandez, Aida A. ; Rodriguez-Kessler, Margarita ; Hernandez-Lucero, Elolsa ; Rosales-Mendoza, Sergio ; Ibanez-Salazar, Alejandro ; Delgado-Sanchez, Pablo ; Jimenez-Bremont, Juan F. / Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance. In: Frontiers in Plant Science. 2015 ; Vol. 5, No. JAN. pp. 1-16.
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Ortega-Amaro, MA, Rodriguez-Hernandez, AA, Rodriguez-Kessler, M, Hernandez-Lucero, E, Rosales-Mendoza, S, Ibanez-Salazar, A, Delgado-Sanchez, P & Jimenez-Bremont, JF 2015, 'Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance', Frontiers in Plant Science, vol. 5, no. JAN, 782, pp. 1-16. https://doi.org/10.3389/fpls.2014.00782

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance. / Ortega-Amaro, Maria A.; Rodriguez-Hernandez, Aida A.; Rodriguez-Kessler, Margarita; Hernandez-Lucero, Elolsa; Rosales-Mendoza, Sergio; Ibanez-Salazar, Alejandro; Delgado-Sanchez, Pablo; Jimenez-Bremont, Juan F.

In: Frontiers in Plant Science, Vol. 5, No. JAN, 782, 20.01.2015, p. 1-16.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

AU - Ortega-Amaro, Maria A.

AU - Rodriguez-Hernandez, Aida A.

AU - Rodriguez-Kessler, Margarita

AU - Hernandez-Lucero, Elolsa

AU - Rosales-Mendoza, Sergio

AU - Ibanez-Salazar, Alejandro

AU - Delgado-Sanchez, Pablo

AU - Jimenez-Bremont, Juan F.

PY - 2015/1/20

Y1 - 2015/1/20

N2 - Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.

AB - Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.

KW - Arabidopsis thaliana

KW - Development

KW - Glycine-rich domain protein

KW - Indole-3-acetic acid

KW - Lactuca sativa

KW - Salt stress

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U2 - 10.3389/fpls.2014.00782

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M3 - Artículo

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JO - Frontiers in Plant Science

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