TY - JOUR
T1 - Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)
AU - Heredia-García, Gerardo
AU - Gómez-Oliván, Leobardo Manuel
AU - Elizalde-Velázquez, Gustavo Axel
AU - Cardoso-Vera, Jesús Daniel
AU - Orozco-Hernández, José Manuel
AU - Rosales-Pérez, Karina Elisa
AU - García-Medina, Sandra
AU - Islas-Flores, Hariz
AU - Galar-Martínez, Marcela
AU - Dublán-García, Octavio
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/8
Y1 - 2022/8
N2 - This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L−1) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC50, EC50 of malformations, and teratogenic index (TI) were 1234.60 ng L−1, 987.77 ng L−1, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.
AB - This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L−1) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC50, EC50 of malformations, and teratogenic index (TI) were 1234.60 ng L−1, 987.77 ng L−1, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.
KW - Bisphenol-A
KW - Embryotoxicity
KW - Oxidative stress, teratogenicity, gene expression, IBR
UR - http://www.scopus.com/inward/record.url?scp=85134674453&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2022.103925
DO - 10.1016/j.etap.2022.103925
M3 - Artículo
C2 - 35835282
AN - SCOPUS:85134674453
SN - 1382-6689
VL - 94
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 103925
ER -