Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)

Gerardo Heredia-García; Leobardo Manuel Gómez-Oliván; Gustavo Axel Elizalde-Velázquez; Jesús Daniel Cardoso-Vera; José Manuel Orozco-Hernández; Karina Elisa Rosales-Pérez; Sandra García-Medina; Hariz Islas-Flores; Marcela Galar-Martínez; Octavio Dublán-García

doi:10.1016/j.etap.2022.103925

Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)

Gerardo Heredia-García, Leobardo Manuel Gómez-Oliván, Gustavo Axel Elizalde-Velázquez, Jesús Daniel Cardoso-Vera, José Manuel Orozco-Hernández, Karina Elisa Rosales-Pérez, Sandra García-Medina, Hariz Islas-Flores, Marcela Galar-Martínez, Octavio Dublán-García

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L⁻¹) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC₅₀, EC₅₀ of malformations, and teratogenic index (TI) were 1234.60 ng L⁻¹, 987.77 ng L⁻¹, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.

Original language	English
Article number	103925
Journal	Environmental Toxicology and Pharmacology
Volume	94
DOIs	https://doi.org/10.1016/j.etap.2022.103925
State	Published - Aug 2022

Keywords

Bisphenol-A
Embryotoxicity
Oxidative stress, teratogenicity, gene expression, IBR

Access to Document

10.1016/j.etap.2022.103925

Cite this

Heredia-García, G., Gómez-Oliván, L. M., Elizalde-Velázquez, G. A., Cardoso-Vera, J. D., Orozco-Hernández, J. M., Rosales-Pérez, K. E., García-Medina, S., Islas-Flores, H., Galar-Martínez, M., & Dublán-García, O. (2022). Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio). Environmental Toxicology and Pharmacology, 94, Article 103925. https://doi.org/10.1016/j.etap.2022.103925

@article{538df23630ef48afaf8e5fcb08e57e49,

title = "Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)",

abstract = "This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L−1) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC50, EC50 of malformations, and teratogenic index (TI) were 1234.60 ng L−1, 987.77 ng L−1, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.",

keywords = "Bisphenol-A, Embryotoxicity, Oxidative stress, teratogenicity, gene expression, IBR",

author = "Gerardo Heredia-Garc{\'i}a and G{\'o}mez-Oliv{\'a}n, {Leobardo Manuel} and Elizalde-Vel{\'a}zquez, {Gustavo Axel} and Cardoso-Vera, {Jes{\'u}s Daniel} and Orozco-Hern{\'a}ndez, {Jos{\'e} Manuel} and Rosales-P{\'e}rez, {Karina Elisa} and Sandra Garc{\'i}a-Medina and Hariz Islas-Flores and Marcela Galar-Mart{\'i}nez and Octavio Dubl{\'a}n-Garc{\'i}a",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = aug,

doi = "10.1016/j.etap.2022.103925",

language = "Ingl{\'e}s",

volume = "94",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

}

Heredia-García, G, Gómez-Oliván, LM, Elizalde-Velázquez, GA, Cardoso-Vera, JD, Orozco-Hernández, JM, Rosales-Pérez, KE, García-Medina, S, Islas-Flores, H, Galar-Martínez, M & Dublán-García, O 2022, 'Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)', Environmental Toxicology and Pharmacology, vol. 94, 103925. https://doi.org/10.1016/j.etap.2022.103925

TY - JOUR

T1 - Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)

AU - Heredia-García, Gerardo

AU - Gómez-Oliván, Leobardo Manuel

AU - Elizalde-Velázquez, Gustavo Axel

AU - Cardoso-Vera, Jesús Daniel

AU - Orozco-Hernández, José Manuel

AU - Rosales-Pérez, Karina Elisa

AU - García-Medina, Sandra

AU - Islas-Flores, Hariz

AU - Galar-Martínez, Marcela

AU - Dublán-García, Octavio

PY - 2022/8

Y1 - 2022/8

N2 - This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L−1) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC50, EC50 of malformations, and teratogenic index (TI) were 1234.60 ng L−1, 987.77 ng L−1, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.

AB - This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L−1) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC50, EC50 of malformations, and teratogenic index (TI) were 1234.60 ng L−1, 987.77 ng L−1, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.

KW - Bisphenol-A

KW - Embryotoxicity

KW - Oxidative stress, teratogenicity, gene expression, IBR

UR - http://www.scopus.com/inward/record.url?scp=85134674453&partnerID=8YFLogxK

U2 - 10.1016/j.etap.2022.103925

DO - 10.1016/j.etap.2022.103925

M3 - Artículo

C2 - 35835282

AN - SCOPUS:85134674453

SN - 1382-6689

VL - 94

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

M1 - 103925

ER -

Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this