Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

Dení Ramírez-Macías; Kristine Shaw; Rocky Ward; Felipe Galván-Magaña; Ricardo Vázquez-Juárez

doi:10.1111/j.1755-0998.2008.02197.x

Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

Dení Ramírez-Macías, Kristine Shaw, Rocky Ward, Felipe Galván-Magaña, Ricardo Vázquez-Juárez

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Resumen

In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

Idioma original	Inglés
Páginas (desde-hasta)	798-800
Número de páginas	3
Publicación	Molecular Ecology Resources
Volumen	9
N.º	3
DOI	https://doi.org/10.1111/j.1755-0998.2008.02197.x
Estado	Publicada - may. 2009
Publicado de forma externa	Sí

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title = "Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)",

abstract = "In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.",

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doi = "10.1111/j.1755-0998.2008.02197.x",

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T1 - Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

AU - Ramírez-Macías, Dení

AU - Shaw, Kristine

AU - Ward, Rocky

AU - Galván-Magaña, Felipe

AU - Vázquez-Juárez, Ricardo

PY - 2009/5

Y1 - 2009/5

N2 - In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

AB - In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

KW - Genomic library

KW - Microsatellites

KW - Rhincodon typus

KW - Rhincodontidae

KW - Whale shark

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U2 - 10.1111/j.1755-0998.2008.02197.x

DO - 10.1111/j.1755-0998.2008.02197.x

M3 - Artículo

SN - 1755-098X

VL - 9

SP - 798

EP - 800

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

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ER -

Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

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