Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

Dení Ramírez-Macías; Kristine Shaw; Rocky Ward; Felipe Galván-Magaña; Ricardo Vázquez-Juárez

doi:10.1111/j.1755-0998.2008.02197.x

Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

Dení Ramírez-Macías, Kristine Shaw, Rocky Ward, Felipe Galván-Magaña, Ricardo Vázquez-Juárez

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

Original language	English
Pages (from-to)	798-800
Number of pages	3
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02197.x
State	Published - May 2009
Externally published	Yes

Keywords

Genomic library
Microsatellites
Rhincodon typus
Rhincodontidae
Whale shark

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10.1111/j.1755-0998.2008.02197.x

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title = "Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)",

abstract = "In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.",

keywords = "Genomic library, Microsatellites, Rhincodon typus, Rhincodontidae, Whale shark",

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T1 - Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

AU - Ramírez-Macías, Dení

AU - Shaw, Kristine

AU - Ward, Rocky

AU - Galván-Magaña, Felipe

AU - Vázquez-Juárez, Ricardo

PY - 2009/5

Y1 - 2009/5

N2 - In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

AB - In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy-Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.

KW - Genomic library

KW - Microsatellites

KW - Rhincodon typus

KW - Rhincodontidae

KW - Whale shark

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DO - 10.1111/j.1755-0998.2008.02197.x

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VL - 9

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EP - 800

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

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ER -

Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)

Abstract

Keywords

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