@article{5c81ee8af4594e128164bb401766215e,
title = "Is an intermediate state populated on the folding pathway of ubiquitin?",
abstract = "In the last couple of years, there has been increasing debate as to the presence and role of intermediate states on the folding pathways of several small proteins, including the 76-residue protein ubiquitin. Here, we present detailed kinetic studies to establish whether an intermediate state is ever populated during the folding of this protein. We show that the differences observed in previous studies are attributable to the transient aggregation of the protein during folding. Using a highly soluble construct of ubiquitin, which does not aggregate during folding, we establish the conditions in which an intermediate state is sufficiently stable to be observed by kinetic measurements.",
keywords = "1-Anilinonaphthalene-8-sulfonate, ANS, 1-anilinonaphthalene-8-sulfonate, Chevron plot, GdmCl, guanidinium chloride, Intermediate, Rollover, Three-state, Two-state",
author = "Went, {Heather M.} and Benitez-Cardoza, {Claudia G.} and Jackson, {Sophie E.}",
note = "Funding Information: We thank Dek Woolfson for kindly providing us with the pCANTABB-WT-UBQ vector, Andy Robertson for providing us with tagless-F45W ubiquitin and many helpful discussions. In addition, we thank Heinrich Roder and Tobin Sosnick for their helpful correspondence. H.M.W. is funded by an EPSRC studentship, C.G.B.-C. acknowledges funding from the DGRI/SEP and CONACyT No. 010092. The work was funded in part by The Royal Society and The Welton Foundation.",
year = "2004",
month = jun,
day = "4",
doi = "10.1016/j.febslet.2004.04.089",
language = "Ingl{\'e}s",
volume = "567",
pages = "333--338",
journal = "FEBS Letters",
issn = "0014-5793",
number = "2-3",
}