Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

Victoria Gabriela Roblero-Bartolón; Elizabeth Maldonado-Alvarado; José Iván Galván-Mendoza; Eva Ramón-Gallegos

doi:10.1063/1.4764623

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

Victoria Gabriela Roblero-Bartolón, Elizabeth Maldonado-Alvarado, José Iván Galván-Mendoza, Eva Ramón-Gallegos

Escuela Nacional de Ciencias Biológicas (ENCB)

Producción científica: Capítulo del libro/informe/acta de congreso › Contribución a la conferencia › revisión exhaustiva

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Resumen

Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

Idioma original	Inglés
Título de la publicación alojada	Medical Physics - Twelfth Mexican Symposium on Medical Physics
Páginas	137-139
Número de páginas	3
DOI	https://doi.org/10.1063/1.4764623
Estado	Publicada - 2012
Evento	12th Mexican Symposium on Medical Physics - Oaxaca, México Duración: 16 mar. 2012 → 19 mar. 2012

Serie de la publicación

Nombre	AIP Conference Proceedings
Volumen	1494
ISSN (versión impresa)	0094-243X
ISSN (versión digital)	1551-7616

Conferencia

Conferencia	12th Mexican Symposium on Medical Physics
País/Territorio	México
Ciudad	Oaxaca
Período	16/03/12 → 19/03/12

ODS de las Naciones Unidas

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10.1063/1.4764623

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@inproceedings{61765f0428dd414b8e794a5068c9ce95,

title = "Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy",

abstract = "Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.",

keywords = "Nanoparticles, PDT, PpIX, cancer",

author = "Roblero-Bartol{\'o}n, {Victoria Gabriela} and Elizabeth Maldonado-Alvarado and Galv{\'a}n-Mendoza, {Jos{\'e} Iv{\'a}n} and Eva Ram{\'o}n-Gallegos",

year = "2012",

doi = "10.1063/1.4764623",

language = "Ingl{\'e}s",

isbn = "9780735411067",

series = "AIP Conference Proceedings",

pages = "137--139",

booktitle = "Medical Physics - Twelfth Mexican Symposium on Medical Physics",

note = "12th Mexican Symposium on Medical Physics ; Conference date: 16-03-2012 Through 19-03-2012",

}

Roblero-Bartolón, VG, Maldonado-Alvarado, E, Galván-Mendoza, JI & Ramón-Gallegos, E 2012, Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy. En Medical Physics - Twelfth Mexican Symposium on Medical Physics. AIP Conference Proceedings, vol. 1494, pp. 137-139, 12th Mexican Symposium on Medical Physics, Oaxaca, México, 16/03/12. https://doi.org/10.1063/1.4764623

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy. / Roblero-Bartolón, Victoria Gabriela; Maldonado-Alvarado, Elizabeth; Galván-Mendoza, José Iván et al.
Medical Physics - Twelfth Mexican Symposium on Medical Physics. 2012. p. 137-139 (AIP Conference Proceedings; Vol. 1494).

Producción científica: Capítulo del libro/informe/acta de congreso › Contribución a la conferencia › revisión exhaustiva

TY - GEN

T1 - Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

AU - Roblero-Bartolón, Victoria Gabriela

AU - Maldonado-Alvarado, Elizabeth

AU - Galván-Mendoza, José Iván

AU - Ramón-Gallegos, Eva

PY - 2012

Y1 - 2012

N2 - Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

AB - Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

KW - Nanoparticles

KW - PDT

KW - PpIX

KW - cancer

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U2 - 10.1063/1.4764623

DO - 10.1063/1.4764623

M3 - Contribución a la conferencia

AN - SCOPUS:84873618088

SN - 9780735411067

T3 - AIP Conference Proceedings

SP - 137

EP - 139

BT - Medical Physics - Twelfth Mexican Symposium on Medical Physics

T2 - 12th Mexican Symposium on Medical Physics

Y2 - 16 March 2012 through 19 March 2012

ER -

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

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