Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

Victoria Gabriela Roblero-Bartolón; Elizabeth Maldonado-Alvarado; José Iván Galván-Mendoza; Eva Ramón-Gallegos

doi:10.1063/1.4764623

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

Victoria Gabriela Roblero-Bartolón, Elizabeth Maldonado-Alvarado, José Iván Galván-Mendoza, Eva Ramón-Gallegos

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

Original language	English
Title of host publication	Medical Physics - Twelfth Mexican Symposium on Medical Physics
Pages	137-139
Number of pages	3
DOIs	https://doi.org/10.1063/1.4764623
State	Published - 2012
Event	12th Mexican Symposium on Medical Physics - Oaxaca, Mexico Duration: 16 Mar 2012 → 19 Mar 2012

Publication series

Name	AIP Conference Proceedings
Volume	1494
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	12th Mexican Symposium on Medical Physics
Country/Territory	Mexico
City	Oaxaca
Period	16/03/12 → 19/03/12

Keywords

Nanoparticles
PDT
PpIX
cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.4764623

Cite this

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title = "Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy",

abstract = "Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.",

keywords = "Nanoparticles, PDT, PpIX, cancer",

author = "Roblero-Bartol{\'o}n, {Victoria Gabriela} and Elizabeth Maldonado-Alvarado and Galv{\'a}n-Mendoza, {Jos{\'e} Iv{\'a}n} and Eva Ram{\'o}n-Gallegos",

year = "2012",

doi = "10.1063/1.4764623",

language = "Ingl{\'e}s",

isbn = "9780735411067",

series = "AIP Conference Proceedings",

pages = "137--139",

booktitle = "Medical Physics - Twelfth Mexican Symposium on Medical Physics",

note = "12th Mexican Symposium on Medical Physics ; Conference date: 16-03-2012 Through 19-03-2012",

}

Roblero-Bartolón, VG, Maldonado-Alvarado, E, Galván-Mendoza, JI & Ramón-Gallegos, E 2012, Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy. in Medical Physics - Twelfth Mexican Symposium on Medical Physics. AIP Conference Proceedings, vol. 1494, pp. 137-139, 12th Mexican Symposium on Medical Physics, Oaxaca, Mexico, 16/03/12. https://doi.org/10.1063/1.4764623

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy. / Roblero-Bartolón, Victoria Gabriela; Maldonado-Alvarado, Elizabeth; Galván-Mendoza, José Iván et al.
Medical Physics - Twelfth Mexican Symposium on Medical Physics. 2012. p. 137-139 (AIP Conference Proceedings; Vol. 1494).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

AU - Roblero-Bartolón, Victoria Gabriela

AU - Maldonado-Alvarado, Elizabeth

AU - Galván-Mendoza, José Iván

AU - Ramón-Gallegos, Eva

PY - 2012

Y1 - 2012

N2 - Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

AB - Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanoparticles coupled to protoporphyrin IX increases the effectiveness of PDT. The aim of this study was to determine the site of accumulation of the conjugate npAu-PpIX in cells of cervical cancer by the use of specific dyes and confocal microscopy. The results indicate that the gold nanoparticles coupled to protoporphyrin IX are accumulated in both the cytoplasm and nucleus of HeLa cells.

KW - Nanoparticles

KW - PDT

KW - PpIX

KW - cancer

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U2 - 10.1063/1.4764623

DO - 10.1063/1.4764623

M3 - Contribución a la conferencia

AN - SCOPUS:84873618088

SN - 9780735411067

T3 - AIP Conference Proceedings

SP - 137

EP - 139

BT - Medical Physics - Twelfth Mexican Symposium on Medical Physics

T2 - 12th Mexican Symposium on Medical Physics

Y2 - 16 March 2012 through 19 March 2012

ER -

Intracellular localization analysis of npAu-PpIX in HeLa cells using specific dyes and confocal microscopy

Abstract

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Conference

Keywords

UN SDGs

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