TY - JOUR
T1 - Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica
AU - Trejo-Tapia, Gabriela
AU - Arias-Castro, Carlos
AU - Rodríguez-Mendiola, Martha
N1 - Funding Information:
G. Trejo-Tapia wishes to thank the National Council of Science and Technology (CONACyT - Mexico) for the scholarship received.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.
AB - The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.
KW - Iron
KW - Lavender
KW - Nitrate
KW - Phosphate
KW - Secondary metabolite
KW - Sucrose
UR - http://www.scopus.com/inward/record.url?scp=0037276844&partnerID=8YFLogxK
U2 - 10.1023/A:1021270907918
DO - 10.1023/A:1021270907918
M3 - Artículo
SN - 0167-6857
VL - 72
SP - 7
EP - 12
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 1
ER -