Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica

Gabriela Trejo-Tapia; Carlos Arias-Castro; Martha Rodríguez-Mendiola

doi:10.1023/A:1021270907918

Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica

Gabriela Trejo-Tapia, Carlos Arias-Castro, Martha Rodríguez-Mendiola

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The effects of the concentration of PO₄^3-, NO₃^-, Fe ²⁺, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO₄^3-, 14.1 mM NO₃^-, 1 mM Fe ²⁺, 30 g l^-1 sucrose and 10 g l^-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe²⁺ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l^-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.

Original language	English
Pages (from-to)	7-12
Number of pages	6
Journal	Plant Cell, Tissue and Organ Culture
Volume	72
Issue number	1
DOIs	https://doi.org/10.1023/A:1021270907918
State	Published - 1 Jan 2003

Keywords

Iron
Lavender
Nitrate
Phosphate
Secondary metabolite
Sucrose

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10.1023/A:1021270907918

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title = "Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica",

abstract = "The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.",

keywords = "Iron, Lavender, Nitrate, Phosphate, Secondary metabolite, Sucrose",

author = "Gabriela Trejo-Tapia and Carlos Arias-Castro and Martha Rodr{\'i}guez-Mendiola",

note = "Funding Information: G. Trejo-Tapia wishes to thank the National Council of Science and Technology (CONACyT - Mexico) for the scholarship received.",

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doi = "10.1023/A:1021270907918",

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AU - Trejo-Tapia, Gabriela

AU - Arias-Castro, Carlos

AU - Rodríguez-Mendiola, Martha

N1 - Funding Information: G. Trejo-Tapia wishes to thank the National Council of Science and Technology (CONACyT - Mexico) for the scholarship received.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.

AB - The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.

KW - Iron

KW - Lavender

KW - Nitrate

KW - Phosphate

KW - Secondary metabolite

KW - Sucrose

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U2 - 10.1023/A:1021270907918

DO - 10.1023/A:1021270907918

M3 - Artículo

SN - 0167-6857

VL - 72

SP - 7

EP - 12

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 1

ER -

Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica

Abstract

Keywords

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