Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica

Gabriela Trejo-Tapia, Carlos Arias-Castro, Martha Rodríguez-Mendiola

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.
Original languageAmerican English
Pages (from-to)7-12
Number of pages5
JournalPlant Cell, Tissue and Organ Culture
DOIs
StatePublished - 1 Jan 2003

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inoculum
Lavandula angustifolia
culture media
pigments
sucrose
cell growth
productivity
viability
cell viability
phosphates
direct current
iron
augmentation

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abstract = "The effects of the concentration of PO43-, NO3-, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (≡L. latifolia Vill.), were studied. The combination of 2.5 mM PO43-, 14.1 mM NO3-, 1 mM Fe 2+, 30 g l-1 sucrose and 10 g l-1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l-1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.",
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Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica. / Trejo-Tapia, Gabriela; Arias-Castro, Carlos; Rodríguez-Mendiola, Martha.

In: Plant Cell, Tissue and Organ Culture, 01.01.2003, p. 7-12.

Research output: Contribution to journalArticle

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AU - Trejo-Tapia, Gabriela

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