TY - JOUR
T1 - Genomic and phenotypic beef quality indicators of Charolais cattle in México
AU - Mejía, Carmen Y.Muñoz
AU - Bracamonte, Gaspar M.Parra
AU - Rincón, Ana M.Sifuentes
AU - González, Juan C.Martínez
AU - Bustamante, Luis A.López
AU - Vera, Williams A.
AU - de la Rosa Reyna, Xochitl F.
PY - 2012/6
Y1 - 2012/6
N2 - Tenderness and marbling are polygenic traits used as indicators of good meat quality. Among different genes related to meat quality, μ-calpaine (CAPN1) and thyroglobulin (TG5) have been specifically linked to tenderness and marbling, respectively. Objectives: to estimate the allelic and genotypic frequencies of markers in CAPN1 and TG5 genes, and relate their presence to beef carcass quality. Methods: CAPN1 and TG5 polymorphisms were identified by PCR-ACRS and PCR-RFLP, respectively, validating their putative effects on beef carcass using real time ultrasound in Charolais candidate sires (n=80). Results: computed genotypic frequencies in CAP4751 and TG5 showed Hardy-Weinberg equilibrium, while CAP316 expressed deviation from equilibrium. Association analysis indicated a significant effect of CAP4751 on rib eye area (REA) (p<0.05) and intramuscular fat (IMF) (p<0.10), while TG5 showed a significant trend on yield grade (YG). Conclusions: these results support the use of these markers for assessing traits related to meat quality, and warrant further studies to validate their use in cattle herds for breeding purposes.
AB - Tenderness and marbling are polygenic traits used as indicators of good meat quality. Among different genes related to meat quality, μ-calpaine (CAPN1) and thyroglobulin (TG5) have been specifically linked to tenderness and marbling, respectively. Objectives: to estimate the allelic and genotypic frequencies of markers in CAPN1 and TG5 genes, and relate their presence to beef carcass quality. Methods: CAPN1 and TG5 polymorphisms were identified by PCR-ACRS and PCR-RFLP, respectively, validating their putative effects on beef carcass using real time ultrasound in Charolais candidate sires (n=80). Results: computed genotypic frequencies in CAP4751 and TG5 showed Hardy-Weinberg equilibrium, while CAP316 expressed deviation from equilibrium. Association analysis indicated a significant effect of CAP4751 on rib eye area (REA) (p<0.05) and intramuscular fat (IMF) (p<0.10), while TG5 showed a significant trend on yield grade (YG). Conclusions: these results support the use of these markers for assessing traits related to meat quality, and warrant further studies to validate their use in cattle herds for breeding purposes.
KW - Calpain
KW - Charolais cattle
KW - PCR-ACRS
KW - PCR-RFLP
KW - Thyroglobulin
UR - http://www.scopus.com/inward/record.url?scp=84864084094&partnerID=8YFLogxK
M3 - Artículo
SN - 0120-0690
VL - 25
SP - 210
EP - 219
JO - Revista Colombiana de Ciencias Pecuarias
JF - Revista Colombiana de Ciencias Pecuarias
IS - 2
ER -