Abstract
Tenderness and marbling are polygenic traits used as indicators of good meat quality. Among different genes related to meat quality, μ-calpaine (CAPN1) and thyroglobulin (TG5) have been specifically linked to tenderness and marbling, respectively. Objectives: to estimate the allelic and genotypic frequencies of markers in CAPN1 and TG5 genes, and relate their presence to beef carcass quality. Methods: CAPN1 and TG5 polymorphisms were identified by PCR-ACRS and PCR-RFLP, respectively, validating their putative effects on beef carcass using real time ultrasound in Charolais candidate sires (n=80). Results: computed genotypic frequencies in CAP4751 and TG5 showed Hardy-Weinberg equilibrium, while CAP316 expressed deviation from equilibrium. Association analysis indicated a significant effect of CAP4751 on rib eye area (REA) (p<0.05) and intramuscular fat (IMF) (p<0.10), while TG5 showed a significant trend on yield grade (YG). Conclusions: these results support the use of these markers for assessing traits related to meat quality, and warrant further studies to validate their use in cattle herds for breeding purposes.
| Translated title of the contribution | Genomic and phenotypic beef quality indicators of Charolais cattle in México |
|---|---|
| Original language | English |
| Pages (from-to) | 210-219 |
| Number of pages | 10 |
| Journal | Revista Colombiana de Ciencias Pecuarias |
| Volume | 25 |
| Issue number | 2 |
| State | Published - Jun 2012 |
Keywords
- Calpain
- Charolais cattle
- PCR-ACRS
- PCR-RFLP
- Thyroglobulin