TY - JOUR
T1 - Hypolipidemic Effect of Avocado (Persea americana Mill) Seed in a Hypercholesterolemic Mouse Model
AU - Pahua-Ramos, María Elena
AU - Ortiz-Moreno, Alicia
AU - Chamorro-Cevallos, Germán
AU - Hernández-Navarro, María Dolores
AU - Garduño-Siciliano, Leticia
AU - Necoechea-Mondragón, Hugo
AU - Hernández-Ortega, Marcela
N1 - Funding Information:
Acknowledgments This research was partly funded by the Consejo Nacional de Ciencia y Tecnología (CONACyT) scholarship, Secretaria de Investigación y Posgrado-IPN Proyect Number. 20100788, Comisión de Operación y Fomento de Actividades Académicas del IPN (COFAA-IPN), and Universidad Autónoma del Estado de México.
PY - 2012/3
Y1 - 2012/3
N2 - Avocado seed contains elevated levels of phenolic compounds and exhibits antioxidant properties. We investigated the effect of Avocado Seed Flour (ASF) on the lipid levels in mice on a hyperlipidemic diet. The concentration of phenols was determined by high-performance liquid chromatography, antioxidant activity was evaluated using the Trolox equivalent antioxidant capacity method, and dietary fiber was measured using the Association of Official Analytical Chemists (AOAC) method. The LD50 of ASF was determined using Lorke's method and hypolipidemic activity was evaluated in a hypercholesterolemic model in mice. Protocatechuic acid was the main phenolic compound found in ASF, followed by kaempferide and vanillic acid. The total phenolic content in the methanolic extract of ASF was 292. 00 ± 9. 81 mg gallic acid equivalents/g seed dry weight and the antioxidant activity resulted in 173. 3 μmol Trolox equivalents/g DW. In addition, a high content of dietary fiber was found (34. 8%). The oral LD50 for ASF was 1767 mg/kg body weight, and treatment with ASF significantly reduced the levels of total cholesterol, LDL-C, and prediction of the atherogenic index. Therefore, the antioxidant activity of phenolic compounds and dietary fiber in ASF may be responsible for the hypocholesterolemic activity of ASF in a hyperlipidemic model of mice.
AB - Avocado seed contains elevated levels of phenolic compounds and exhibits antioxidant properties. We investigated the effect of Avocado Seed Flour (ASF) on the lipid levels in mice on a hyperlipidemic diet. The concentration of phenols was determined by high-performance liquid chromatography, antioxidant activity was evaluated using the Trolox equivalent antioxidant capacity method, and dietary fiber was measured using the Association of Official Analytical Chemists (AOAC) method. The LD50 of ASF was determined using Lorke's method and hypolipidemic activity was evaluated in a hypercholesterolemic model in mice. Protocatechuic acid was the main phenolic compound found in ASF, followed by kaempferide and vanillic acid. The total phenolic content in the methanolic extract of ASF was 292. 00 ± 9. 81 mg gallic acid equivalents/g seed dry weight and the antioxidant activity resulted in 173. 3 μmol Trolox equivalents/g DW. In addition, a high content of dietary fiber was found (34. 8%). The oral LD50 for ASF was 1767 mg/kg body weight, and treatment with ASF significantly reduced the levels of total cholesterol, LDL-C, and prediction of the atherogenic index. Therefore, the antioxidant activity of phenolic compounds and dietary fiber in ASF may be responsible for the hypocholesterolemic activity of ASF in a hyperlipidemic model of mice.
KW - Avocado
KW - Dietary
KW - Fiber
KW - Hypolipidemic
KW - Phenolic compounds
KW - Seed
UR - http://www.scopus.com/inward/record.url?scp=84858270966&partnerID=8YFLogxK
U2 - 10.1007/s11130-012-0280-6
DO - 10.1007/s11130-012-0280-6
M3 - Artículo
SN - 0921-9668
VL - 67
SP - 10
EP - 16
JO - Plant Foods for Human Nutrition
JF - Plant Foods for Human Nutrition
IS - 1
ER -