TY - JOUR
T1 - Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes
AU - Gutiérrez-Salinas, José
AU - Morales-González, José A.
AU - Madrigal-Santillán, Eduardo
AU - Esquivel-Soto, Jaime
AU - Esquivel-Chirino, César
AU - García-Luna, Manuel
AU - González-Rubio, G. R.
AU - Domínguez, Sigrit Suástegui
AU - Valadez-Vega, Carmen
PY - 2010/9
Y1 - 2010/9
N2 - Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strength and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks.
AB - Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strength and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks.
KW - Apoptosis
KW - Caspase-3
KW - Leukocytes
KW - Sodium fluoride
KW - bcl-2
KW - p53
UR - http://www.scopus.com/inward/record.url?scp=77958506727&partnerID=8YFLogxK
U2 - 10.3390/ijms11093610
DO - 10.3390/ijms11093610
M3 - Artículo
C2 - 20957113
SN - 1661-6596
VL - 11
SP - 3610
EP - 3622
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
ER -