Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes

José Gutiérrez-Salinas, José A. Morales-González, Eduardo Madrigal-Santillán, Jaime Esquivel-Soto, César Esquivel-Chirino, Manuel García-Luna, G. R. González-Rubio, Sigrit Suástegui Domínguez, Carmen Valadez-Vega

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strengt h and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks. © 2010 by the authors; license MDPI, Basel, Switzerland.
Original languageAmerican English
Pages (from-to)3610-3622
Number of pages13
JournalInternational Journal of Molecular Sciences
DOIs
StatePublished - 1 Sep 2010
Externally publishedYes

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sodium fluorides
Sodium Fluoride
leukocytes
apoptosis
Cell death
rats
Rats
Leukocytes
Sodium
Apoptosis
Fluorides
Metabolism
Bone
Animals
metabolism
Coal
bones
fluorides
animals
Air

Cite this

Gutiérrez-Salinas, José ; Morales-González, José A. ; Madrigal-Santillán, Eduardo ; Esquivel-Soto, Jaime ; Esquivel-Chirino, César ; García-Luna, Manuel ; González-Rubio, G. R. ; Domínguez, Sigrit Suástegui ; Valadez-Vega, Carmen. / Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes. In: International Journal of Molecular Sciences. 2010 ; pp. 3610-3622.
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title = "Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes",
abstract = "Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strengt h and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks. {\circledC} 2010 by the authors; license MDPI, Basel, Switzerland.",
author = "Jos{\'e} Guti{\'e}rrez-Salinas and Morales-Gonz{\'a}lez, {Jos{\'e} A.} and Eduardo Madrigal-Santill{\'a}n and Jaime Esquivel-Soto and C{\'e}sar Esquivel-Chirino and Manuel Garc{\'i}a-Luna and Gonz{\'a}lez-Rubio, {G. R.} and Dom{\'i}nguez, {Sigrit Su{\'a}stegui} and Carmen Valadez-Vega",
year = "2010",
month = "9",
day = "1",
doi = "10.3390/ijms11093610",
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Gutiérrez-Salinas, J, Morales-González, JA, Madrigal-Santillán, E, Esquivel-Soto, J, Esquivel-Chirino, C, García-Luna, M, González-Rubio, GR, Domínguez, SS & Valadez-Vega, C 2010, 'Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes', International Journal of Molecular Sciences, pp. 3610-3622. https://doi.org/10.3390/ijms11093610

Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes. / Gutiérrez-Salinas, José; Morales-González, José A.; Madrigal-Santillán, Eduardo; Esquivel-Soto, Jaime; Esquivel-Chirino, César; García-Luna, Manuel; González-Rubio, G. R.; Domínguez, Sigrit Suástegui; Valadez-Vega, Carmen.

In: International Journal of Molecular Sciences, 01.09.2010, p. 3610-3622.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Exposure to sodium fluoride produces signs of apoptosis in rat leukocytes

AU - Gutiérrez-Salinas, José

AU - Morales-González, José A.

AU - Madrigal-Santillán, Eduardo

AU - Esquivel-Soto, Jaime

AU - Esquivel-Chirino, César

AU - García-Luna, Manuel

AU - González-Rubio, G. R.

AU - Domínguez, Sigrit Suástegui

AU - Valadez-Vega, Carmen

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strengt h and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks. © 2010 by the authors; license MDPI, Basel, Switzerland.

AB - Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strengt h and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in the expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks. © 2010 by the authors; license MDPI, Basel, Switzerland.

U2 - 10.3390/ijms11093610

DO - 10.3390/ijms11093610

M3 - Article

SP - 3610

EP - 3622

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

ER -