TY - JOUR
T1 - Evaluation of nanomechanical properties of tomato root by atomic force microscopy
AU - Nicolás-Álvarez, D. E.
AU - Andraca-Adame, J. A.
AU - Chanona-Pérez, J. J.
AU - Méndez-Méndez, J. V.
AU - Cárdenas-Pérez, S.
AU - Rodríguez-Pulido, A.
N1 - Publisher Copyright:
Copyright © Microscopy Society of America 2019.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Here, different tissue surfaces of tomato root were characterized employing atomic force microscopy on day 7 and day 21 of growth through Young's modulus and plasticity index. These parameters provide quantitative information regarding the mechanical behavior of the tomato root under fresh conditions in different locations of the cross-section of root [cell surface of the epidermis, parenchyma (Pa), and vascular bundles (Vb)]. The results show that the mechanical parameters depend on the indented region, tissue type, and growth time. Thereby, the stiffness increases in the cell surface of epidermal tissue with increasing growth time (from 9.19 ± 0.68 to 13.90 ± 1.68 MPa) and the cell surface of Pa tissue displays the opposite behavior (from 1.74 ± 0.49 to 0.48 ± 0.55); the stiffness of cell surfaces of Vb tissue changes from 10.60 ± 0.58 to 6.37 ± 0.53 MPa, all cases showed a statistical difference (p < 0.05). Viscoelastic behavior dominates the mechanical forces in the tomato root. The current study is a contribution to a better understanding of the cell mechanics behavior of different tomato root tissues during growth.
AB - Here, different tissue surfaces of tomato root were characterized employing atomic force microscopy on day 7 and day 21 of growth through Young's modulus and plasticity index. These parameters provide quantitative information regarding the mechanical behavior of the tomato root under fresh conditions in different locations of the cross-section of root [cell surface of the epidermis, parenchyma (Pa), and vascular bundles (Vb)]. The results show that the mechanical parameters depend on the indented region, tissue type, and growth time. Thereby, the stiffness increases in the cell surface of epidermal tissue with increasing growth time (from 9.19 ± 0.68 to 13.90 ± 1.68 MPa) and the cell surface of Pa tissue displays the opposite behavior (from 1.74 ± 0.49 to 0.48 ± 0.55); the stiffness of cell surfaces of Vb tissue changes from 10.60 ± 0.58 to 6.37 ± 0.53 MPa, all cases showed a statistical difference (p < 0.05). Viscoelastic behavior dominates the mechanical forces in the tomato root. The current study is a contribution to a better understanding of the cell mechanics behavior of different tomato root tissues during growth.
KW - Young's modulus
KW - atomic force microscopy
KW - growth stages
KW - nanoindentation
KW - plasticity index
KW - tomato root tissues
UR - http://www.scopus.com/inward/record.url?scp=85068532861&partnerID=8YFLogxK
U2 - 10.1017/S1431927619014636
DO - 10.1017/S1431927619014636
M3 - Artículo
C2 - 31272515
SN - 1431-9276
VL - 25
SP - 989
EP - 997
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
IS - 4
ER -