TY - JOUR
T1 - Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum
AU - Pacheco, Neith
AU - Larralde-Corona, C. Patricia
AU - Sepulveda, Jose
AU - Trombotto, Stéphan
AU - Domard, Alain
AU - Shirai, Keiko
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (MW) chitosans displayed higher inhibitory activity against the yeast than low MW biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low MW and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.
AB - Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (MW) chitosans displayed higher inhibitory activity against the yeast than low MW biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low MW and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.
KW - Chitosan
KW - Penicillium digitatum
KW - Pichia guillermondii
UR - http://www.scopus.com/inward/record.url?scp=44649103927&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2007.10.009
DO - 10.1016/j.ijbiomac.2007.10.009
M3 - Artículo
C2 - 18031804
SN - 0141-8130
VL - 43
SP - 20
EP - 26
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - 1
ER -