Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum

Neith Pacheco; C. Patricia Larralde-Corona; Jose Sepulveda; Stéphan Trombotto; Alain Domard; Keiko Shirai

doi:10.1016/j.ijbiomac.2007.10.009

Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum

Neith Pacheco, C. Patricia Larralde-Corona, Jose Sepulveda, Stéphan Trombotto, Alain Domard, Keiko Shirai

Centro de Biotecnología Genómica (CBG)

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (M_W) chitosans displayed higher inhibitory activity against the yeast than low M_W biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low M_W and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.

Original language	English
Pages (from-to)	20-26
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/j.ijbiomac.2007.10.009
State	Published - 1 Jul 2008

Keywords

Chitosan
Penicillium digitatum
Pichia guillermondii

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10.1016/j.ijbiomac.2007.10.009

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title = "Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum",

abstract = "Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (MW) chitosans displayed higher inhibitory activity against the yeast than low MW biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low MW and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.",

keywords = "Chitosan, Penicillium digitatum, Pichia guillermondii",

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T1 - Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum

AU - Pacheco, Neith

AU - Larralde-Corona, C. Patricia

AU - Sepulveda, Jose

AU - Trombotto, Stéphan

AU - Domard, Alain

AU - Shirai, Keiko

PY - 2008/7/1

Y1 - 2008/7/1

N2 - Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (MW) chitosans displayed higher inhibitory activity against the yeast than low MW biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low MW and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.

AB - Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (MW) chitosans displayed higher inhibitory activity against the yeast than low MW biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low MW and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.

KW - Chitosan

KW - Penicillium digitatum

KW - Pichia guillermondii

UR - http://www.scopus.com/inward/record.url?scp=44649103927&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2007.10.009

DO - 10.1016/j.ijbiomac.2007.10.009

M3 - Artículo

C2 - 18031804

SN - 0141-8130

VL - 43

SP - 20

EP - 26

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 1

ER -

Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum

Abstract

Keywords

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