TY - JOUR
T1 - Contenido agave salmiana syrup improves the production of recombinant human interleukin-2 in escherichia coli volumen 8, número 3, 2009 / volume 8, number 3, 2009
AU - Balderas-Hernández, V. E.
AU - Medina-Rivero, E.
AU - Barba-De la Rosa, A. P.
AU - De León-Rodríguez, A.
N1 - Publisher Copyright:
© 2021, Universidad Autonoma Metropolitana. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The expression of heterologousintemperizadosproteinseninsuelosEscherichiay sedimentoscoli is strongly affected by the type of carbon source used. In this work, the expression of a synthetic codon-optimized gene of human interleukin-2 in E. coli BL21-SI, carrying plasmid pET12a-rhIL2 (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil is presented. Glucose, fructose or Agave syrup from Agave salmiana, were used as carbon sources for production of recombinant human IL-2 (rhIL-2) in 1.5-Land sediments)bioreactor aerobic cultures using mineral medium. Codon optimization of the native hIL-2 gene eliminated the presence ofS.A.35Medina-Moreno,rare codons forS. E.Huerta-Ochoa,coli, and improvedC.A. Lucho-Constantino,the codon usageL. upAguilera-Vázquez,to 76% comparedA. Jiménez-with the native gene sequence. Cultures using 10 g/L glucose showed the lowest production of rhIL-2, and in contrast, cultures using fructose González y M. Gutiérrez-Rojas improved the production of rhIL-2 1.9-times. The utilization of fructose from Agave syrup enhanced the rhIL-2 production 3.9-times in comparison259withCrecimiento,culturessobrevivenciausing glucose,y adaptaciónreaching 103.42±6.61de Bifidobacteriummg infantis a condiciones ácidasIL-2 /L. Also, the specific rhIL-2 production rate (5.52±0.33 mgIL-2 /gDCW·h) and the biomass production (3.09±0.04 g (Growth, survival and adaptation of BifidobacteriumDCW/L) using Agave syrup were the highest observed. infantis to acidic conditions) These results indicate that Agave syrup is an effective carbon source that stimulates the production of rhIL-2 and biomass. This research shows the potentialL. Mayorga-Reyes,utilization of AgavesP. Bustamante-Camilo,to generate alternativeA. Gutiérrez-Nava,and valuableE.biotechnologicalBarranco-Floridoproductsy A. Azaola-along with the alcoholic beverages. Espinosa.
AB - The expression of heterologousintemperizadosproteinseninsuelosEscherichiay sedimentoscoli is strongly affected by the type of carbon source used. In this work, the expression of a synthetic codon-optimized gene of human interleukin-2 in E. coli BL21-SI, carrying plasmid pET12a-rhIL2 (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil is presented. Glucose, fructose or Agave syrup from Agave salmiana, were used as carbon sources for production of recombinant human IL-2 (rhIL-2) in 1.5-Land sediments)bioreactor aerobic cultures using mineral medium. Codon optimization of the native hIL-2 gene eliminated the presence ofS.A.35Medina-Moreno,rare codons forS. E.Huerta-Ochoa,coli, and improvedC.A. Lucho-Constantino,the codon usageL. upAguilera-Vázquez,to 76% comparedA. Jiménez-with the native gene sequence. Cultures using 10 g/L glucose showed the lowest production of rhIL-2, and in contrast, cultures using fructose González y M. Gutiérrez-Rojas improved the production of rhIL-2 1.9-times. The utilization of fructose from Agave syrup enhanced the rhIL-2 production 3.9-times in comparison259withCrecimiento,culturessobrevivenciausing glucose,y adaptaciónreaching 103.42±6.61de Bifidobacteriummg infantis a condiciones ácidasIL-2 /L. Also, the specific rhIL-2 production rate (5.52±0.33 mgIL-2 /gDCW·h) and the biomass production (3.09±0.04 g (Growth, survival and adaptation of BifidobacteriumDCW/L) using Agave syrup were the highest observed. infantis to acidic conditions) These results indicate that Agave syrup is an effective carbon source that stimulates the production of rhIL-2 and biomass. This research shows the potentialL. Mayorga-Reyes,utilization of AgavesP. Bustamante-Camilo,to generate alternativeA. Gutiérrez-Nava,and valuableE.biotechnologicalBarranco-Floridoproductsy A. Azaola-along with the alcoholic beverages. Espinosa.
KW - 265 Statisticalfructose
KW - ApproachNaClto inducer
KW - Codon optimization
KW - Fermentationsyntheticby gene.Sacch
KW - Optimizationrecombinantof ethanolprotein
UR - http://www.scopus.com/inward/record.url?scp=85099534646&partnerID=8YFLogxK
U2 - 10.24275/rmiq/Bio2004
DO - 10.24275/rmiq/Bio2004
M3 - Artículo
AN - SCOPUS:85099534646
SN - 1665-2738
VL - 20
SP - 399
EP - 412
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 1
ER -