Contenido agave salmiana syrup improves the production of recombinant human interleukin-2 in escherichia coli volumen 8, número 3, 2009 / volume 8, number 3, 2009

The expression of heterologousintemperizadosproteinseninsuelosEscherichiay sedimentoscoli is strongly affected by the type of carbon source used. In this work, the expression of a synthetic codon-optimized gene of human interleukin-2 in E. coli BL21-SI, carrying plasmid pET12a-rhIL2 (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil is presented. Glucose, fructose or Agave syrup from Agave salmiana, were used as carbon sources for production of recombinant human IL-2 (rhIL-2) in 1.5-Land sediments)bioreactor aerobic cultures using mineral medium. Codon optimization of the native hIL-2 gene eliminated the presence ofS.A.35Medina-Moreno,rare codons forS. E.Huerta-Ochoa,coli, and improvedC.A. Lucho-Constantino,the codon usageL. upAguilera-Vázquez,to 76% comparedA. Jiménez-with the native gene sequence. Cultures using 10 g/L glucose showed the lowest production of rhIL-2, and in contrast, cultures using fructose González y M. Gutiérrez-Rojas improved the production of rhIL-2 1.9-times. The utilization of fructose from Agave syrup enhanced the rhIL-2 production 3.9-times in comparison259withCrecimiento,culturessobrevivenciausing glucose,y adaptaciónreaching 103.42±6.61de Bifidobacteriummg infantis a condiciones ácidas_IL-2 /L. Also, the specific rhIL-2 production rate (5.52±0.33 mg_IL-2 /g_DCW·h) and the biomass production (3.09±0.04 g (Growth, survival and adaptation of Bifidobacterium_DCW/L) using Agave syrup were the highest observed. infantis to acidic conditions) These results indicate that Agave syrup is an effective carbon source that stimulates the production of rhIL-2 and biomass. This research shows the potentialL. Mayorga-Reyes,utilization of AgavesP. Bustamante-Camilo,to generate alternativeA. Gutiérrez-Nava,and valuableE.biotechnologicalBarranco-Floridoproductsy A. Azaola-along with the alcoholic beverages. Espinosa.