TY - JOUR
T1 - Dystroglycan depletion inhibits the functions of differentiated HL-60 cells
AU - Martínez-Zárate, Alma Delia
AU - Martínez-Vieyra, Ivette
AU - Alonso-Rangel, Lea
AU - Cisneros, Bulmaro
AU - Winder, Steve J.
AU - Cerecedo, Doris
N1 - Funding Information:
This work was supported by CONACYT -México, grant no. 161781 . We thank the Unidad de Microscopía Confocal (Centro de Investigación y Estudios Avanzados del IPN) for technical assistance.
PY - 2014/6/6
Y1 - 2014/6/6
N2 - Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.
AB - Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.
KW - Differentiation
KW - Dystroglycan
KW - HL-60 cells
KW - Migration
KW - Phagocytosis
KW - Respiratory burst activity
UR - http://www.scopus.com/inward/record.url?scp=84901407600&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2014.04.110
DO - 10.1016/j.bbrc.2014.04.110
M3 - Artículo
C2 - 24792180
SN - 0006-291X
VL - 448
SP - 274
EP - 280
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -